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Tipo:	Artigo de Periódico
Título:	Urocortin 2 promotes hypertrophy and enhances skeletal muscle function through cAMP and insulin/IGF-1 signaling pathways
Autor(es):	Natalia Lautherbach Isis C. Kettelhut Dawit Albieiro Pinheiro Gonçalves Wilian A. Silveira Sílvia Paula-Gomes Rafael Rossi Valentim Neuza M. Zanon Marcelo G. Pereira Elen H. Miyabara Luiz C. C. Navegantes
Resumo:	Objective Although it is well established that urocortin 2 (Ucn2), a peptide member of the corticotrophin releasing factor (CRF) family, and its specific corticotrophin-releasing factor 2 receptor (CRF2R) are highly expressed in skeletal muscle, the role of this peptide in the regulation of skeletal muscle mass and protein metabolism remains elusive. Methods To elucidate the mechanisms how Ucn2 directly controls protein metabolism in skeletal muscles of normal mice, we carried out genetic tools, physiological and molecular analyses of muscles in vivo and in vitro. Results Here, we demonstrated that Ucn2 overexpression activated cAMP signaling and promoted an expressive muscle hypertrophy associated with higher rates of protein synthesis and activation of Akt/mTOR and ERK1/2 signaling pathways. Furthermore, Ucn2 induced a decrease in mRNA levels of atrogin-1 and in autophagic flux inferred by an increase in the protein content of LC3-I, LC3-II and p62. Accordingly, Ucn2 reduced both the transcriptional activity of FoxO in vivo and the overall protein degradation in vitro through an inhibition of lysosomal proteolytic activity. In addition, we demonstrated that Ucn2 induced a fast-to-slow fiber type shift and improved fatigue muscle resistance, an effect that was completely blocked in muscles co-transfected with mitogen-activated protein kinase phosphatase 1 (MKP-1), but not with dominant-negative Akt mutant (Aktmt). Conclusions These data suggest that Ucn2 triggers an anabolic and anti-catabolic response in skeletal muscle of normal mice probably through the activation of cAMP cascade and participation of Akt and ERK1/2 signaling. These findings open new perspectives in the development of therapeutic strategies to cope with the loss of muscle mass.
Assunto:	Hipertrofia Fadiga Proteínas
Idioma:	eng
País:	Brasil
Editor:	Universidade Federal de Minas Gerais
Sigla da Instituição:	UFMG
Departamento:	EEF - DEPARTAMENTO DE EDUCAÇÃO FÍSICA EEFFTO - ESCOLA DE EDUCAÇÃO FISICA, FISIOTERAPIA E TERAPIA OCUPACIONAL
Tipo de Acesso:	Acesso Aberto
Identificador DOI:	https://doi.org/10.1016/j.molmet.2022.101492
URI:	http://hdl.handle.net/1843/64803
Data do documento:	2022
metadata.dc.url.externa:	https://www.sciencedirect.com/science/article/pii/S2212877822000618?via%3Dihub
metadata.dc.relation.ispartof:	Molecular Metabolism
Aparece nas coleções:	Artigo de Periódico

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