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http://hdl.handle.net/1843/65618| Tipo: | Artigo de Periódico |
| Título: | Nearest-neighbour parameters optimized for melting temperature prediction of DNA/RNA hybrids at high and low salt concentrations |
| Autor(es): | Vivianne Basilio Barbosa Erik de Oliveira Martins Gerald Weber |
| Resumo: | Gene editing technologies sparked a renewed interest in the hybridization of DNA/RNA duplexes, yet little improvement on nearest-neighbour parameters was made over the past two decades. For low sodium concentration no parameter set was yet calculated. Here, we revised the existing experimental datasets and used an expanded set of sequences from which we recalculated the nearest-neighbour parameters, reducing the average temperature prediction uncertainty to 1.6 °C. Two experimental sets using temperatures extracted via different methods were used with similar results, with the curve-fitting method achieving a slight advantage in prediction quality over other methods. Additionally, we obtained new parameters for low salt with an average uncertainty of 0.98 °C. We also tested several types of salt correction factors and concluded that it is advisable to use those originally developed for RNA/RNA rather than for DNA/DNA. |
| Assunto: | DNA Ácido ribonucleico |
| Idioma: | eng |
| País: | Brasil |
| Editor: | Universidade Federal de Minas Gerais |
| Sigla da Instituição: | UFMG |
| Departamento: | ICX - DEPARTAMENTO DE FÍSICA |
| Tipo de Acesso: | Acesso Restrito |
| Identificador DOI: | https://doi.org/10.1016/j.bpc.2019.106189 |
| URI: | http://hdl.handle.net/1843/65618 |
| Data do documento: | 2019 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0301462219301814?via%3Dihub |
| metadata.dc.relation.ispartof: | Biophysical Chemistry |
| Aparece nas coleções: | Artigo de Periódico |
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