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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/66317
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dc.creatorPriscila Valera Guerrapt_BR
dc.creatorTatiani Uceli Maioliipt_BR
dc.creatorAna Maria Caetano Fariapt_BR
dc.creatorCláudia Ida Brodskynpt_BR
dc.creatorCamila Mattos Andradept_BR
dc.creatorIvanéia Valeriano Nunespt_BR
dc.creatorBrena Cardoso Gamapt_BR
dc.creatorRafael Tibúrciopt_BR
dc.creatorWashington Luis Conrado Santospt_BR
dc.creatorVasco Ariston de Carvalho Azevedopt_BR
dc.creatorNatalia Machado Tavarespt_BR
dc.creatorJuliana de Souza Rebouçaspt_BR
dc.date.accessioned2024-03-21T14:19:18Z-
dc.date.available2024-03-21T14:19:18Z-
dc.date.issued2021-06-23-
dc.citation.volume12pt_BR
dc.citation.spage1010010pt_BR
dc.citation.epage15pt_BR
dc.identifier.doihttps://doi.org/10.3389/fimmu.2021.647987pt_BR
dc.identifier.issn1664-3224pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/66317-
dc.description.resumoCutaneous leishmaniasis caused by L. braziliensis induces a pronounced Th1 inflammatory response characterized by IFN-γ production. Even in the absence of parasites, lesions result from a severe inflammatory response in which inflammatory cytokines play an important role. Different approaches have been used to evaluate the therapeutic potential of orally administrated heat shock proteins (Hsp). These proteins are evolutionarily preserved from bacteria to humans, highly expressed under inflammatory conditions and described as immunodominant antigens. Tolerance induced by the oral administration of Hsp65 is capable of suppressing inflammation and inducing differentiation in regulatory cells, and has been successfully demonstrated in several experimental models of autoimmune and inflammatory diseases. We initially administered recombinant Lactococcus lactis (L. lactis) prior to infection as a proof of concept, in order to verify its immunomodulatory potential in the inflammatory response arising from L. braziliensis. Using this experimental approach, we demonstrated that the oral administration of a recombinant L. lactis strain, which produces and secretes Hsp65 from Mycobacterium leprae directly into the gut, mitigated the effects of inflammation caused by L. braziliensis infection in association or not with PAM 3CSK4 (N-α-Palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-L-cysteine, a TLR2 agonist). This was evidenced by the production of anti-inflammatory cytokines and the expansion of regulatory T cells in the draining lymph nodes of BALB/c mice. Our in vitro experimental results suggest that IL-10, TLR-2 and LAP are important immunomodulators in L. braziliensis infection. In addition, recombinant L. lactis administered 4 weeks after infection was observed to decrease lesion size, as well as the number of parasites, and produced a higher IL-10 production and decrease IFN-γ secretion. Together, these results indicate that Hsp65-producing L. lactis can be considered as an alternative candidate for treatment in both autoimmune diseases, as well as in chronic infections that cause inflammatory disease.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentENF - DEPARTAMENTO DE NUTRIÇÃOpt_BR
dc.publisher.departmentENFERMAGEM - ESCOLA DE ENFERMAGEMpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofFrontiers in Immunologypt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectLeishmania braziliensispt_BR
dc.subjectHeat shock protein 65pt_BR
dc.subjectLactococcus lactispt_BR
dc.subjectOral tolerancept_BR
dc.subjectIL-10pt_BR
dc.subjectTLR2pt_BR
dc.subjectTGF-bpt_BR
dc.subject.otherLeishmania braziliensispt_BR
dc.subject.otherResposta ao Choque Térmicopt_BR
dc.subject.otherLactococcus lactispt_BR
dc.subject.otherInterleucina-10pt_BR
dc.subject.otherReceptor 2 Toll-Likept_BR
dc.subject.otherFator de Crescimento Transformador betapt_BR
dc.titleOral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infectionpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.647987/fullpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7538-3208pt_BR
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