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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/67389
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dc.creatorCristiane Bigatti Pereirapt_BR
dc.creatorNívea Pereira de Sápt_BR
dc.creatorBeatriz Martins Borellipt_BR
dc.creatorCarlos Augusto Rosapt_BR
dc.creatorPaulo Jorge Sanches Barbeirapt_BR
dc.creatorBetania Barros Cotapt_BR
dc.creatorSusana Johannpt_BR
dc.date.accessioned2024-04-18T13:50:32Z-
dc.date.available2024-04-18T13:50:32Z-
dc.date.issued2016-
dc.citation.volume100pt_BR
dc.citation.spage205pt_BR
dc.citation.epage212pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.micpath.2016.09.022pt_BR
dc.identifier.issn0882-4010pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/67389-
dc.description.resumoThe antifungal effects of two eicosanoic acids, 2-amino-3,4-dihydroxy-2-25-(hydroxymethyl)-14-oxo-6,12-eicosenoic acid (compound 1) and myriocin (compound 2), isolated from Mycosphaerella sp. were evaluated against Cryptococcus neoformans and C. gattii. The compounds displayed antifungal activities against several isolates of C. neoformans and C. gattii, with minimal inhibitory concentration (MIC) values ranging from 0.49 to 7.82 μM for compound 1 and 0.48-1.95 μM for compound 2. In the checkerboard microtiter test, both compounds exhibited synergistic activity with amphotericin B against C. gattii. Ultrastructural analysis revealed several signs of damage in C. gattii and C. neoformans cells treated with compounds 1 and 2, including deformities in cell shape, depressions on the surface, and withered cells. The cells of C. gattii treated with compounds 1 and 2 showed less loss of cellular material in comparison to those treated with amphotericin B. The difference in cellular material loss increased in a test compound concentration-dependent manner. Consistent with this observation, compounds 1 and 2 were able to internalize propidium iodide (PI) in C. gattii cells. In addition, compound 2 induced the formation of several pseudohyphae, suggesting that it could reduce virulence in C. gattii cells. The study results show that these natural products led to membrane damage; however, this may not be the main target of action. These compounds have potential antifungal activity and could be useful in further studies for developing more effective combination therapies with amphotericin B and reducing side effects in patients.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIApt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE QUÍMICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofMicrobial Pathogenesispt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectCryptococcuspt_BR
dc.subjectMembrane damagept_BR
dc.subjectMycosphaerellapt_BR
dc.subjectSynergismpt_BR
dc.subject.otherFungos - Biotecnologiapt_BR
dc.subject.otherMembranas (Biologia)pt_BR
dc.subject.otherFungos do solopt_BR
dc.subject.otherAtividade antifúngicapt_BR
dc.subject.otherFungos patogênicospt_BR
dc.subject.otherTremellalespt_BR
dc.titleAntifungal activity of eicosanoic acids isolated from the endophytic fungus Mycosphaerella sp. against Cryptococcus neoformans and C. gattiipt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S0882401016304740pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9966-1321pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0056-9075pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0770-8680pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0041-2043pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8068-1720pt_BR
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