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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/67451
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dc.creatorNívea Pereira de Sápt_BR
dc.creatorPatrícia Silva Cisalpinopt_BR
dc.creatorCaryne Margotto Bertollopt_BR
dc.creatorPatrícia Campi Santospt_BR
dc.creatorCarlos Augusto Rosapt_BR
dc.creatorDaniele da Glória de Souzapt_BR
dc.creatorPaulo Jorge Sanches Barbeirapt_BR
dc.creatorTânia Maria de Almeida Alvespt_BR
dc.creatorCarlos Leomar Zanipt_BR
dc.creatorSusana Johannpt_BR
dc.date.accessioned2024-04-19T12:53:37Z-
dc.date.available2024-04-19T12:53:37Z-
dc.date.issued2019-
dc.citation.volume57pt_BR
dc.citation.issue3pt_BR
dc.citation.spage332pt_BR
dc.citation.epage339pt_BR
dc.identifier.doihttps://doi.org/10.1093/mmy/myy045pt_BR
dc.identifier.issn1460-2709pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/67451-
dc.description.resumoParacoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin American countries. Amphotericin B, sulfonamides, and azoles may be used in the treatment of PCM. However, the high toxicity, prolonged course of treatment, and significant frequency of disease relapse compromise their use. Therefore, there is a need to seek new therapeutic options. We conducted tests with thiosemicarbazone of lapachol (TSC-lap) to determine the antifungal activity and phenotypic effects against several isolates of Paracoccidioides spp. In addition, we evaluated the toxicity against murine macrophages and the ability to enhance phagocytosis. Further, we verified that TSC-lap was active against yeasts but did not show any interaction with the drugs tested. The TSC-lap showed no toxicity at the concentration of 40 μg/ml in macrophages, and at 15.6 μg/ml it could increase the phagocytic index. We observed that this compound induced in vitro ultrastructural changes manifested as withered and broken cells beyond a disorganized cytoplasm with accumulation of granules. We did not observe indications of activity in the cell wall, although membrane damages were noted. We observed alterations in the membrane permeability, culminating in a significant increase in K+ efflux and a gradual loss of the cellular content with increase in the concentration of TSC-lap. In addition, we showed a significant reduction of ergosterol amount in the Pb18 membrane. These data reinforce the possible mechanism of action of this compound to be closely associated with ergosterol biosynthesis and reaffirms the antifungal potential of TSC-lap against Paracoccidioidesspp.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIApt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE QUÍMICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofMedical Mycologypt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectAntifungalpt_BR
dc.subjectErgosterolpt_BR
dc.subjectThiosemicarbazone of lapacholpt_BR
dc.subjectParacoccidioidespt_BR
dc.subject.otherFungospt_BR
dc.subject.otherCelulas - Membranaspt_BR
dc.subject.otherAntimicoticopt_BR
dc.subject.otherAtividade antifúngicapt_BR
dc.subject.otherParacoccidioides brasiliensispt_BR
dc.subject.otherMicoses sistêmicaspt_BR
dc.subject.otherProdutos de ação antimicrobianapt_BR
dc.titleThiosemicarbazone of lapachol acts on cell membrane in Paracoccidioides brasiliensispt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://academic.oup.com/mmy/article/57/3/332/5045352pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4115-559Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0056-9075pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7478-5934pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0770-8680pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0582-3968pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-1859-177Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8068-1720pt_BR
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