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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/69774
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dc.creatorTiago Coelho de Assis Lagept_BR
dc.creatorSérgio Antônio Fernandespt_BR
dc.creatorLuzia Valentina Modolopt_BR
dc.creatorThamilla Maria Silva Macielpt_BR
dc.creatorYane Campolina Cachuite Motapt_BR
dc.creatorFrancesca Sistopt_BR
dc.creatorJosé Ricardo Sabinopt_BR
dc.creatorJosué Carinhanha Caldas Santospt_BR
dc.creatorIsis Martins Figueiredopt_BR
dc.creatorCarla Masiapt_BR
dc.creatorÂngelo de Fátimapt_BR
dc.date.accessioned2024-07-05T21:13:00Z-
dc.date.available2024-07-05T21:13:00Z-
dc.date.issued2018-
dc.citation.volume42pt_BR
dc.citation.spage5356pt_BR
dc.citation.epage5366pt_BR
dc.identifier.doihttps://doi.org/10.1039/C8NJ00072Gpt_BR
dc.identifier.issn1144-0546pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/69774-
dc.description.resumoThe interaction of (S)-(−)-usnic acid (2) and fumarprotocetraric acid (3), isolated from Cladonia rappii (lichen), and commercial (R)-(+)-usnic acid (1) with urease was investigated in vitro by molecular spectroscopy at pH 7.4 and kinetics experiments using jack bean type III urease. All lichen compounds tested interact with urease by a statistical quenching mechanism forming non-fluorescent complexes that change the native protein structure. Formation of complexes was spontaneous and stabilized mainly by electrostatic forces, in which the interaction magnitude was determined to be 3 < 2 < 1. Compound 2, whose tridimensional structure is disclosed here, acts as a mixed inhibitor while compounds 1 and 3 function as competitive ones. The (R)-(+)-UA (1) is the most efficient lichen metabolite with respect to impairment of the growth of five H. pylori strains. The minimum inhibitory concentrations (MIC) for the lichen metabolites tested were lower (from 2- to 7.8-fold) than those of omeprazole (reference drug) against all H. pylori strains tested. Overall, the lichen metabolites 1–3 are promising lead compounds for the design of more efficient urease inhibitors for the treatment of H. pylori infections.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE QUÍMICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofNew Journal of Chemistrypt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectUreasept_BR
dc.subjectUrease inhibitorspt_BR
dc.subjectHelicobacter pyloript_BR
dc.subjectLichen natural productspt_BR
dc.subjectUsnic acidpt_BR
dc.subjectMolecular spectroscopypt_BR
dc.subject.otherQuímicapt_BR
dc.subject.otherBioquímicapt_BR
dc.subject.otherHelicobacter pyloript_BR
dc.subject.otherUreasept_BR
dc.subject.otherProdutos naturaispt_BR
dc.subject.otherEspectroscopia molecularpt_BR
dc.titleIn vitro inhibition of helicobacter pylori and interaction studies of lichen natural products with jack bean ureasept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://pubs.rsc.org/en/content/articlelanding/2018/nj/c8nj00072g#!pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0007-5216pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-3054-3316pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-8033-0434pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9477-1919pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2853-2215pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0166-2164pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-3332-9967pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9525-5123pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1873-5342pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-1875-4217pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2344-5590pt_BR
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