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http://hdl.handle.net/1843/71565| Type: | Artigo de Periódico |
| Title: | Anti-inflammatory mechanisms and pharmacological actions of phycocyanobilin in a mouse model of experimental autoimmune encephalomyelitis: a therapeutic promise for multiple sclerosis |
| Authors: | Javier Marín-Prida Julio Raúl Fernández-Massó Ignacio Hernández-González Gillian Martínez-Donato Gerardo Guillén-Nieto Eduardo Pentón-Arias Mauro Martins Teixeira Giselle Pentón-Rol Nancy Pavón-Fuentes Nielsen Lagumersindez-Denis Hanlet Camacho-Rodríguez Ana Margarita García-Soca Rocío de la Caridad Sarduy-Chávez Érica Leandro Marciano Vieira Juliana Carvalho Tavares Viviana Falcón-Cama |
| Abstract: | Cytokines, demyelination and neuroaxonal degeneration in the central nervous system are pivotal elements implicated in the pathogenesis of multiple sclerosis (MS) and its nonclinical model of experimental autoimmune encephalomyelitis (EAE). Phycocyanobilin (PCB), a chromophore of the biliprotein C-Phycocyanin (C-PC) from Spirulina platensis, has antioxidant, immunoregulatory and anti-inflammatory effects in this disease, and it could complement the effect of other Disease Modifying Treatments (DMT), such as Interferon-β (IFN-β). Here, our main goal was to evaluate the potential PCB benefits and its mechanisms of action to counteract the chronic EAE in mice. MOG35-55-induced EAE was implemented in C57BL/6 female mice. Clinical signs, pro-inflammatory cytokines levels by ELISA, qPCR in the brain and immunohistochemistry using precursor/mature oligodendrocytes cells antibodies in the spinal cord, were assessed. PCB enhanced the neurological condition, and waned the brain concentrations of IL-17A and IL-6, pro-inflammatory cytokines, in a dose-dependent manner. A down- or up-regulating activity of PCB at 1 mg/kg was identified in the brain on three (LINGO1, NOTCH1, and TNF-α), and five genes (MAL, CXCL12, MOG, OLIG1, and NKX2-2), respectively. Interestingly, a reduction of demyelination, active microglia/macrophages density, and axonal damage was detected along with an increase in oligodendrocyte precursor cells and mature oligodendrocytes, when assessed the spinal cords of EAE mice that took up PCB. The studies in vitro in rodent encephalitogenic T cells and in vivo in the EAE mouse model with the PCB/IFN-β combination, showed an enhanced positive effect of this combined therapy. Overall, these results demonstrate the anti-inflammatory activity and the protective properties of PCB on the myelin and support its use with IFN-β as an improved DMT combination for MS. |
| Subject: | Citocinas Esclerose Múltipla Encefalomielite Autoimune Experimental Remielinização Interferon beta |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | 10.3389/fimmu.2022.1036200 |
| URI: | http://hdl.handle.net/1843/71565 |
| Issue Date: | 3-Nov-2022 |
| metadata.dc.url.externa: | https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.1036200/full |
| metadata.dc.relation.ispartof: | Frontiers in Immunology |
| Appears in Collections: | Artigo de Periódico |
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