Imunolocalização de proteínas que participam da sinalização intracelular no tecido lamelar de cavalos submetidos ao modelo de indução de laminite oligofrutose

Abstract

Laminitis in horses remains a therapeutic challenge due to the scarcity of effective options for prevention and treatment, despite efforts to understand its pathophysiology. This study investigated the expression of various proteins, including Rictor, Raptor, p-STAT3, p-RPS6, and calprotectin, to identify potential therapeutic targets. Lamellar tissue samples from horses with oligofructose-induced laminitis were analyzed, revealing significant correlations between the inflammatory proteins calprotectin and p-STAT3, as well as between these and p-RPS6. The analysis showed robust correlations between calprotectin and p-STAT3 in different layers of lamellar tissue, indicating a direct association between inflammation and activation of cellular signaling pathways. Furthermore, a positive correlation was observed between p-RPS6 and calprotectin, suggesting an interaction between inflammation and RPS6 phosphorylation, a key protein in the mTOR pathway. The association between Raptor and p-RPS6, p-STAT3, and calprotectin in the sublamella suggests the involvement of the mTOR pathway in the pathogenesis of laminitis, especially considering its relationship with interleukin-6 (IL-6)- induced inflammation, a factor known to play a crucial role in the inflammatory response. These results suggest that the activation of the mTOR/RPS6 and IL-6/gp130 pathways may be critical in the progression of sepsis-related laminitis in horses. These findings highlight the potential of the mTOR/RPS6 and IL-6/gp130 cellular pathways as therapeutic targets in equine laminitis. By understanding the interactions between key proteins and signaling pathways, there is an opportunity to develop targeted therapeutic strategies to modulate these processes and mitigate the effects of sepsis-associated laminitis in horses. This research offers valuable insights for the development of more effective and specific treatments for laminitis in horses, aiming not only to mitigate inflammation and tissue damage but also to preserve the structural and functional integrity of lamellar tissue, thereby improving the prognosis and quality of life of these animals.