Hematologia e bioquímicas em Araras Canindé (Ara ararauna), tigre d’água (Trachemys scripta) e pacamãs (Lophiosilurus alexandri) sob efeito de diferentes anticoagulantes

Abstract

Studies in the field of haematology of wild and exotic animals are an important tool to assist clinical diagnosis and allow a better understanding of the characteristics and particularities of these animals. The present study aims to describe values and aspects of the haematology and biochemistry of Canindé macaws (Ara ararauna), water tigers (Trachemys scripta spp) and pacamãs (Lophiosilurus alexandri), under the effect of three anticoagulants used in veterinary clinical routine: EDTA -K3, sodium citrate and heparin. Blood samples were collected from sixty animals of each species, 3 to 5mL of blood from each animal, the volume varied depending on the species. After collection, the blood was distributed into four microtubes containing: (1) 10% EDTA-K3; (2) 3.8% sodium citrate; (3) sodium heparin. The remainder of the sample was placed in a micro tube, without anticoagulant. Blood smears were prepared for all samples, determining the haematocrit value and protein concentration. Samples were aliquoted to count red blood cells, leukocytes, and thrombocytes in the Neubauer chamber and after centrifugation to obtain plasma and serum for processing biochemical evaluations. The following analytes were measured by colorimetric technique: albumin, total proteins, urea, creatinine, glucose, alanine aminotransferase, aspartate aminotransferase, uric acid, cholesterol, triglycerides, lactate dehydrogenase, and the others, by kinetic method: phosphorus, calcium and magnesium, in an automatic biochemistry device in each anticoagulant studied and in the serum. Based on the results, it is concluded that for macaws EDTA is the best anticoagulant for performing the blood count; sodium citrate and heparin are similar in evaluating the hematimetry of macaws, resulting in values lower than those obtained in samples with EDTA; In biochemical analyses, EDTA proved to be valid for a greater number of biochemical analyses than sodium citrate and heparin and that EDTA is the anticoagulant of choice for carrying out haematological and biochemical analyses of Canindé macaws. The results from water tigers indicate that sodium citrate is the best anticoagulant for performing a blood count; Among the anticoagulants analysed, sodium citrate proved to be valid for a greater number of biochemical analyses than EDTA and heparin; sodium citrate is the anticoagulant of choice for carrying out haematological and biochemical analyses of Trachemys. For pacamãs, the results indicate that for haematological evaluation, the choice of sodium citrate as an anticoagulant will provide the best result; Among the anticoagulants analysed, sodium citrate proved to be valid for a greater number of biochemical analyses (5 analytes) than sodium citrate and sodium heparin (3 analytes); Sodium citrate is the anticoagulant of choice for carrying out haematological and biochemical analyses of pacamãs.