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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/76719
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dc.creatorDouglas Santos Gonçalvespt_BR
dc.creatorDenys Matheus Santana Costa Souzapt_BR
dc.creatorDulcinéia de Carvalhopt_BR
dc.creatorLeandro Silva de Oliveirapt_BR
dc.creatorGustavo Leal Teixeirapt_BR
dc.creatorGilvano Ebling Brondanipt_BR
dc.date.accessioned2024-09-20T11:37:35Z-
dc.date.available2024-09-20T11:37:35Z-
dc.date.issued2023-05-
dc.citation.volume3pt_BR
dc.citation.spage100024pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.bamboo.2023.100024pt_BR
dc.identifier.issn2773-1391pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/76719-
dc.description.resumoFast-growing forest species with multiple uses, like bamboo, have aroused interest for their silvicultural applications. Bamboo species are a valuable source of renewable raw material, and Bambusa vulgaris is an economically important species. However, there are limitations to large-scale cloning of adult-selected genotypes. This study aimed to evaluate the in vitro cloning of Bambusa vulgaris in different culture systems, sucrose and activated charcoal supplementation by the inter-simple-sequence repeat (ISSR) molecular markers. In vitro bud multiplication and shoot elongation were evaluated in three cultivation systems: semi-solid and liquid culture media, and temporary immersion bioreactor (TIB). The sucrose concentrations, 0 and 30 g L−1 were evaluated in the stages. Both the culture media were supplemented with 2.0 mg L−1 benzylaminopurine (BAP) and 0.5 mg L−1 α-naphthalene acetic acid (NAA). The absence and presence of activated charcoal (100 mg L−1) were evaluated in the in vitro rooting. MS culture medium was supplemented with 2 mg L−1 indole-3-butyric acid (IBA), 1.0 mg L−1 NAA, and 0.5 mg L−1 BAP. Semi-solid culture medium supplemented with 30 g L−1 of sucrose presented superior emission of bud per explant. Liquid culture medium supplemented with 30 g L−1 of sucrose presented the most elongated shoots. Activated charcoal in the culture medium did not influence the adventitious rooting. Micropropagated plants showed genetic fidelity and were clones of the adult selected plant.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofAdvances in Bamboo Science-
dc.rightsAcesso Abertopt_BR
dc.subject.otherBambupt_BR
dc.subject.otherGenética vegetalpt_BR
dc.subject.otherBiorreatorespt_BR
dc.subject.otherCarbono ativadopt_BR
dc.titleIn vitro cloning of Bambusa vulgaris Schrad. ex J. C. Wendl.: Effect of culture systems, sucrose and activated charcoal supplementationpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S2773139123000101pt_BR
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