Avaliação do polimorfismo 4g/5g do PAI-1 em pacientes com acidente vascular encefálico isquêmico e seus subtipos

Abstract

PAI-1 (Plasminogen Activator Inhibitor-1) is a glycoprotein that belongs to the serpin family that are the main inhibitor of plasminogen activators ofthe tissue type (tPA) and urokinase type (uPA). Increased levels of PAI-1 affects the fibrinolytic system and influences the fibrin clot and may be associated with the development of thromboembolic diseases such as myocardial infarction and stroke, however its action in this last is still controversial. The PAI-1 gene has several polymorphic loci, including a 4G/5G insertion/deletion at 675 bp from the start of the promoter. It has been reported that individuals with 4G/4G genotype have significantly higher plasma PAI-1 levels (about 25% higher) than individuals with others genotypes (4G/5G and 5G/5G).Due to few studies correlating the polymorphism of the PAI-1 gene with the susceptibility and the etiology of stroke, we compared the genotype of 4G/5G polymorphism distribution in the different stroke subtypes.We conducted venous blood collection of patients admitted for follow-up post-stroke at the Neurology Service of Santa Casa de Belo Horizonte (All n=144; 43 atherothrombotic, 39 cardioembolic, 40lacunar and 22 cryptogenic) and the venous blood collection of controls (n=112) was performed in the medical specialties of the Santa Casa. The control group and patients were adjusted for age, gender, hypertension, diabetes, and smoking. The polymorphismanalysis were performed by polymerase chain reaction (PCR). Statistical analysis was performed for comparison between groups. Significant difference between groups will be considered for p<0.05.We correlate the polymorphism between patients/controls and with susceptibility to different stroke etiologies and were not found significant differences in both (p>0.05). These results suggest that the PAI-1 polymorphism unrelated to the risk of stroke as well as the susceptibility to any of the subtypes. These results are supported by other studies.