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Type:	Artigo de Periódico
Title:	Guidelines for the purification and characterization of extracellularvesicles of parasites
Authors:	Carmen Fernandez Becerra Nuria Cortes Serra Paula Abou Karam Paula Meneghetti Rafael Pedro Madeira Ziv Porat Rodrigo Soares Adriana Oliveira Costa Sima Rafati Anabela Cordeiro-da-Silva Nuno Santarém Patricia Xander Christopher Fernandez-Prada Daniel Alfandari George Dong Iris Aparici Irit Rosenhek-Goldian Mehrdad Shokouhy Melisa Gualdron-lopez Nicholy Lozano Lysangela Alves Hernando del Portillo Obando Melisa Gualdrón-López Ana Claudia Torrecilhas Sergio Schenkman Martin Olivier Igor Almeida Marcel Ramirez Dolores Bernal Antonio Marcilla Neta Regev-Rudzki
Abstract:	Parasites are responsible for the most neglected tropical diseases, affecting over a bil-lion people worldwide (WHO, 2015) and accounting for billions of cases a year andresponsible for several millions of deaths. Research on extracellular vesicles (EVs)has increased in recent years and demonstrated that EVs shed by pathogenic para-sites interact with host cells playing an important role in the parasite’s survival, suchas facilitation of infection, immunomodulation, parasite adaptation to the host envi-ronment and the transfer of drug resistance factors. Thus, EVs released by parasitesmediate parasite-parasite and parasite-host intercellular communication. In addi-tion, they are being explored as biomarkers of asymptomatic infections and diseaseprognosis after drug treatment. However, most current protocols used for the iso-lation, size determination, quantification and characterization of molecular cargo ofEVs lack greater rigor, standardization, and adequate quality controls to certify theenrichment or purity of the ensuing bioproducts. We are now initiating major guide-lines based on the evolution of collective knowledge in recent years. The main pointscovered in this position paper are methods for the isolation and molecular character-ization of EVs obtained from parasite-infected cell cultures, experimental animals,and patients. The guideline also includes a discussion of suggested protocols andfunctional assays in host cells
Subject:	Doenças parasitárias Helminto Protozoário patogênico Infecção
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.10.1002/jex2.117
URI:	http://hdl.handle.net/1843/79486
Issue Date:	19-Oct-2023
metadata.dc.url.externa:	https://isevjournals.onlinelibrary.wiley.com/doi/full/10.1002/jex2.117
metadata.dc.relation.ispartof:	Journal of Extracellular Biology
Appears in Collections:	Artigo de Periódico

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