1. Repositório Institucional da UFMG
2. Publicações Científicas e Culturais
3. Artigo de Periódico

Type:	Artigo de Periódico
Title:	Subcutaneous model for the study of dengue virus infection in immune competent mice
Authors:	Beatriz Senra Santos Natalia Lima Pessoa Natalia Lucinda Gustavo Cardoso de Oliveira Thais Souza Silva Ketyllen Reis Andrade Bruno Galvão Filho Marcele Neves Rocha Alexandre Vieira Machado Pedro Augusto Alves Érica Alessandra Rocha Alves Erna Geessien Kroon Marco Antônio Campos
Abstract:	Various mouse models to study dengue have been described by different authors, some of them using immunodeficient or some using humanized mice. Our group reported previously a deadly murine model, which used the intracranial inoculum of highly virulent Dengue virus (DENV) in immune competent mouse. Here we present a model of immune competent mouse (C57BL/6), infected subcutaneously by the same highly virulent DENV (DENV3 genotype I). In this immunocompetent systemic mice model, the cytokine levels and hematological parameters such as total and differential leukocyte and platelets counts, together with weight loss, were considered important monitoring parameters, allowing a better understanding of the systemic human disease. Mice were inoculated subcutaneously and evaluated by the percentage weight variation as well as the clinical signs. Hematological parameters and cytokines levels were measured and viral titration in brain tissue or serum neutralization was performed to confirm mice infection. The subcutaneously DENV inoculated mice showed weight loss after infection, but they did not show any other clinical signs. The leukocytes and platelets decreased after subcutaneous inoculation. The cytokines TNF alpha and IFN gamma increased after infection in mice. The subcutaneous model provided scope for improved understanding of the dengue pathogenesis, as well as possible mechanism for protection to subsequent mouse infected by intracranial route in mice. This model could be used to study the vertebrate immune response and evaluation of drugs or vaccine against dengue virus.
Subject:	Citocinas Dengue
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ICB - DEPARTAMENTO DE MICROBIOLOGIA
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.4236/jbm.2018.65011
URI:	http://hdl.handle.net/1843/79844
Issue Date:	24-May-2018
metadata.dc.url.externa:	https://www.scirp.org/pdf/JBM_2018052315022911.pdf
metadata.dc.relation.ispartof:	Journal of Biosciences and Medicines
Appears in Collections:	Artigo de Periódico

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.