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http://hdl.handle.net/1843/79899| Type: | Artigo de Periódico |
| Title: | Proof of concept of a novel multiepitope recombinant protein for the serodiagnosis of patients with chagas disease |
| Authors: | Juliana Martins Machado Isabela Amorim Gonçalves Pereira Ana Clara Gontijo Maia Mariana Ferraz Chaves Francisco Lais Moreira Nogueira Isadora Braga Gandra Anna Julia Ribeiro Kamila Alves Silva Carlos Ananias Aparecido Resende Jonatas Oliveira da Silva Michelli dos Santos Ana Alice Maia Gonçalves Grasiele de Sousa Vieira Tavares Miguel Angel Chávez-Fumagalli Mariana Campos-da-Paz Rodolfo Cordeiro Giunchetti Manoel Otávio da Costa Rocha Ana Thereza Chaves Eduardo Antônio Ferraz Coelho Alexsandro Sobreira Galdino |
| Abstract: | Chagas disease remains a neglected disease that is considered to be a public health problem. The early diagnosis of cases is important to improve the prognosis of infected patients and prevent transmission. Serological tests are the method of choice for diagnosis. However, two serological tests are currently recommended to confirm positive cases. In this sense, more sensitive and specific serological tests need to be developed to overcome these current diagnosis problems. This study aimed to develop a new recombinant multiepitope protein for the diagnosis of Chagas disease, hereafter named rTC. The rTC was constructed based on amino acid sequences from different combinations of Trypanosoma cruzi antigens in the same polypeptide and tested using an enzyme-linked immunosorbent assay (ELISA) to detect different types of Chagas disease. rTC was able to discriminate between indeterminate (IND) and cardiac (CARD) cases and cross-reactive diseases, as well as healthy samples, with 98.28% sensitivity and 96.67% specificity, respectively. These data suggest that rTC has the potential to be tested in future studies against a larger serological panel for the diagnosis of Chagas disease. |
| Subject: | Doença de Chagas Proteínas Recombinantes Saúde pública Diagnóstico de laboratório |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICB - DEPARTAMENTO DE MORFOLOGIA MED - DEPARTAMENTO DE CLÍNICA MÉDICA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.3390/pathogens12020312 |
| URI: | http://hdl.handle.net/1843/79899 |
| Issue Date: | 14-Feb-2023 |
| metadata.dc.url.externa: | https://www.mdpi.com/2076-0817/12/2/312 |
| metadata.dc.relation.ispartof: | Pathogens |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Proof of concept of a novel multiepitope recombinant protein for the serodiagnosis of patients with Chagas Disease.pdf | 2.16 MB | Adobe PDF | View/Open |
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