Please use this identifier to cite or link to this item:
http://hdl.handle.net/1843/80160| Type: | Artigo de Periódico |
| Title: | Yellow fever virus genotyping tool and investigation of suspected adverse events following yellow fever vaccination |
| Authors: | Izabela Maurício de Rezende Pedro Augusto Alves Matheus Soares Arruda Andreza Parreiras Gonçalves Gabriela Fernanda Garcia Oliveira Leonardo Soares Pereira Maria Rita Teixeira Dutra Ana Carolina Campi-Azevedo Valéria Valim Renata Tourinho Santos Jaquelline Germano de Oliveira Carlos Eduardo Calzavara Rodrigo Fabiano do Carmo Said Erna Geessien Kroon Olindo Assis Martins-Filho Andrea Teixeira-Carvalho Betânia Paiva Drumond |
| Abstract: | The yellow fever (YF) vaccine consists of an attenuated virus, and despite its relative safety, some adverse events following YF vaccination have been described. At the end of 2016, Brazil experienced the most massive sylvatic yellow fever outbreak over the last 70 years and an intense campaign of YF vaccination occurred in Minas Gerais state in Southeast Brazil from 2016 to 2018. The present study aimed to develop a genotyping tool and investigate 21 cases of suspected adverse events following YF vaccination. Initial in silico analyses were performed using partial NS5 nucleotide sequences to verify the discriminatory potential between wild-type and vaccine viruses. Samples from patients were screened for the presence of the YFV RNA, using 50UTR as the target, and then used for amplification of partial NS5 gene amplification, sequencing, and phylogenetic analysis. Genotyping indicated that 17 suspected cases were infected by the wild-type yellow fever virus, but four cases remained inconclusive. The genotyping tool was efficient in distinguishing the vaccine from wild-type virus, and it has the potential to be used for the differentiation of all yellow fever virus genotypes. |
| Subject: | Febre amarela Vírus da Febre Amarela Vacinação Imunização Infecções por Arbovirus Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICB - DEPARTAMENTO DE MICROBIOLOGIA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.3390/vaccines7040206 |
| URI: | http://hdl.handle.net/1843/80160 |
| Issue Date: | 4-Dec-2019 |
| metadata.dc.url.externa: | https://www.mdpi.com/2076-393X/7/4/206 |
| metadata.dc.relation.ispartof: | Vaccines |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Yellow fever virus genotyping tool and investigation of suspected adverse events following yellow fever vaccination.pdf | 1.36 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.