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Use este identificador para citar o ir al link de este elemento: http://hdl.handle.net/1843/81833
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Campo DCValorIdioma
dc.creatorSheyla Omonte Nevespt_BR
dc.creatorLuísa Mourão Dias Magalhãespt_BR
dc.creatorJôice Dias Corrêapt_BR
dc.creatorWalderez Ornelas Dutrapt_BR
dc.creatorKenneth John Gollobpt_BR
dc.creatorTarcília Aparecida da Silvapt_BR
dc.creatorMartinho Campolina Rebello Hortapt_BR
dc.creatorPaulo Eduardo Alencar Souzapt_BR
dc.date.accessioned2025-04-24T21:29:14Z-
dc.date.available2025-04-24T21:29:14Z-
dc.date.issued2019-
dc.citation.volume27pt_BR
dc.citation.spagee20180529pt_BR
dc.identifier.doihttps://doi.org/10.1590/1678-7757-2018-0529pt_BR
dc.identifier.issn1678-7765pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/81833-
dc.description.resumoObjectives: Dental composites release unreacted resin monomers into the oral environment, even after polymerization. Periodontal cells are, therefore, exposed to substances that potentially elicit the immune inflammatory response. The underlying molecular mechanisms associated with the interaction between resin monomers and human immune cells found in the gingival crevicular fluid are not fully understood yet. This study investigated the ability of bisphenol A-glycidyl methacrylate (BISGMA), urethane dimethacrylate (UDMA) and triethylene glycol dimethacrylate (TEGDMA) to induce apoptosis and cytokine release by human leukocytes stimulated with a periodontal pathogen. Methodology: Peripheral blood mononuclear cells (PBMC) from 16 healthy individuals were included in this study. To determine the toxicity, the PBMC were incubated for 20 hours, with monomers, for the analysis of cell viability using MTT assay. To evaluate cell death in the populations of monocytes and lymphocytes, they were exposed to sub-lethal doses of each monomer and of heat-inactivated Porphyromonas gingivalis (P. gingivalis) for 5 hours. Secretions of IL-1β, IL-6, IL-10 and TNF-α were determined by ELISA after 20 hours. Results: UDMA and TEGDMA induced apoptosis after a short-time exposure. Bacterial challenge induced significant production of IL-1β and TNF-α (p<0.05). TEGDMA reduced the bacterial-induced release of IL-1β and TNF-α, whereas UDMA reduced IL-1β release (p<0.05). These monomers did not affect IL-10 and IL-6 secretion. BISGMA did not significantly interfere in cytokine release. Conclusions: These results show that resin monomers are toxic to PBMC in a dose-dependent manner, and may influence the local immune inflammatory response and tissue damage mechanisms via regulation of bacterial-induced IL-1β and TNF-α secretion by PBMC.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of applied oral sciencept_BR
dc.rightsAcesso Abertopt_BR
dc.subjectCytokinespt_BR
dc.subjectPorphyromonas gingivalispt_BR
dc.subjectMononuclear leukocytespt_BR
dc.subjectMaterials testingpt_BR
dc.subjectComposite resinspt_BR
dc.subject.otherCytokinespt_BR
dc.subject.otherPorphyromonas gingivalispt_BR
dc.subject.otherLeukocytes, mononuclearpt_BR
dc.subject.otherMaterials testingpt_BR
dc.subject.otherComposite resinspt_BR
dc.subject.otherApoptosispt_BR
dc.subject.otherBisphenol A-glycidyl methacrylatept_BR
dc.subject.otherInterleukin-1betapt_BR
dc.subject.otherTumor necrosis factor-alphapt_BR
dc.subject.otherBacteriapt_BR
dc.subject.otherToxicitypt_BR
dc.subject.otherCell survivalpt_BR
dc.titleComposite-derived monomers affect cell viability and cytokine expression in human leukocytes stimulated with porphyromonas gingivalispt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.scielo.br/j/jaos/a/hzhxR4pwGY7ZzCcjzJbWPHk/?format=pdf&lang=enpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9957-3295pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0170-6163pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7586-9996pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-4184-3867pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9623-7835pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0192-5614pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5166-1982pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-3924-8356pt_BR
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