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http://hdl.handle.net/1843/81833| Tipo: | Artigo de Periódico |
| Título: | Composite-derived monomers affect cell viability and cytokine expression in human leukocytes stimulated with porphyromonas gingivalis |
| Autor(es): | Sheyla Omonte Neves Luísa Mourão Dias Magalhães Jôice Dias Corrêa Walderez Ornelas Dutra Kenneth John Gollob Tarcília Aparecida da Silva Martinho Campolina Rebello Horta Paulo Eduardo Alencar Souza |
| Resumen: | Objectives: Dental composites release unreacted resin monomers into the oral environment, even after polymerization. Periodontal cells are, therefore, exposed to substances that potentially elicit the immune inflammatory response. The underlying molecular mechanisms associated with the interaction between resin monomers and human immune cells found in the gingival crevicular fluid are not fully understood yet. This study investigated the ability of bisphenol A-glycidyl methacrylate (BISGMA), urethane dimethacrylate (UDMA) and triethylene glycol dimethacrylate (TEGDMA) to induce apoptosis and cytokine release by human leukocytes stimulated with a periodontal pathogen. Methodology: Peripheral blood mononuclear cells (PBMC) from 16 healthy individuals were included in this study. To determine the toxicity, the PBMC were incubated for 20 hours, with monomers, for the analysis of cell viability using MTT assay. To evaluate cell death in the populations of monocytes and lymphocytes, they were exposed to sub-lethal doses of each monomer and of heat-inactivated Porphyromonas gingivalis (P. gingivalis) for 5 hours. Secretions of IL-1β, IL-6, IL-10 and TNF-α were determined by ELISA after 20 hours. Results: UDMA and TEGDMA induced apoptosis after a short-time exposure. Bacterial challenge induced significant production of IL-1β and TNF-α (p<0.05). TEGDMA reduced the bacterial-induced release of IL-1β and TNF-α, whereas UDMA reduced IL-1β release (p<0.05). These monomers did not affect IL-10 and IL-6 secretion. BISGMA did not significantly interfere in cytokine release. Conclusions: These results show that resin monomers are toxic to PBMC in a dose-dependent manner, and may influence the local immune inflammatory response and tissue damage mechanisms via regulation of bacterial-induced IL-1β and TNF-α secretion by PBMC. |
| Asunto: | Cytokines Porphyromonas gingivalis Leukocytes, mononuclear Materials testing Composite resins Apoptosis Bisphenol A-glycidyl methacrylate Interleukin-1beta Tumor necrosis factor-alpha Bacteria Toxicity Cell survival |
| Idioma: | eng |
| País: | Brasil |
| Editor: | Universidade Federal de Minas Gerais |
| Sigla da Institución: | UFMG |
| Departamento: | FAO - DEPARTAMENTO DE CLÍNICA ICB - DEPARTAMENTO DE MORFOLOGIA |
| Tipo de acceso: | Acesso Aberto |
| Identificador DOI: | https://doi.org/10.1590/1678-7757-2018-0529 |
| URI: | http://hdl.handle.net/1843/81833 |
| Fecha del documento: | 2019 |
| metadata.dc.url.externa: | https://www.scielo.br/j/jaos/a/hzhxR4pwGY7ZzCcjzJbWPHk/?format=pdf&lang=en |
| metadata.dc.relation.ispartof: | Journal of applied oral science |
| Aparece en las colecciones: | Artigo de Periódico |
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| archivo | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| Composite-derived monomers affect cell viability and cytokine expression in human leukocytes stimulated with Porphyromonas gingivalis.pdf | 2.38 MB | Adobe PDF | Visualizar/Abrir |
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