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http://hdl.handle.net/1843/82193| Type: | Artigo de Periódico |
| Title: | Nestin and neuron-glial antigen 2 transgenes unveil progenitor units in murine salivary glands |
| Authors: | Isabella Bittencourt do Valle Natália Aparecida Gomes Ivana Márcia Alves Diniz José Alcides Almeida de Arruda Tatiana Fernandes Araújo Almeida Mariana Souza Santos Alexander Birbrair Sandra Ventorin Von Zeidler Tarcília Aparecida da Silva |
| Abstract: | Objective: Undifferentiated cells play pivotal roles in sustaining tissue homeostasis during physiological turnovers and after tissue impairment. Nestin and Neuron-glial antigen 2 (NG2) are markers frequently deployed to distinguish progenitor populations. In the salivary gland scenario, these markers remain largely unknown. Particularly for a double-labeled group of progenitor cells (NG2+Nestin+), their phenotype and distribution have never been explored in freshly isolated tissues. Herein, we analyzed a subset of plastic cells that express Nestin and NG2 near the ducts and in the periacinar region of the major salivary glands of murine samples. Design: The major salivary glands tissues of Nestin-GFP/NG2-DsRed mice were analyzed under a fluorescence microscope. The cells marked by GFP and DsRed were counted in the merged image component of random representative images obtained for each gland sample at × 20 magnification. Results: In the parotid, submandibular, and sublingual glands, the population of cells exclusively expressing Nestin was more abundant. There was a predominance of Nestin, NG2, and double-labeled cells in the submandibular gland compared to the parotid gland, mainly near the ductal system. Of note, the sublingual and parotid glands had similar populations of Nestin+ and NG2+, especially in acini, and some positive cells were observed surrounding ducts. |
| Subject: | Nestin Salivary glands Parotid gland Stem cells Sublingual gland Submandibular gland. Submandibular gland Homeostasis |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE CLÍNICA FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA ICB - DEPARTAMENTO DE PATOLOGIA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1016/j.archoralbio.2021.105344 |
| URI: | http://hdl.handle.net/1843/82193 |
| Issue Date: | Feb-2022 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0003996921003071?via%3Dihub |
| metadata.dc.relation.ispartof: | Archives of Oral Biology |
| Appears in Collections: | Artigo de Periódico |
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