Please use this identifier to cite or link to this item:
http://hdl.handle.net/1843/83279| Type: | Artigo de Periódico |
| Title: | The role of 5-lipoxygenase in Aggregatibacter actinomycetemcomitans-induced alveolar bone loss |
| Authors: | Mila Fernandes Moreira Madeira Danielle Glória Souza Celso Martins Queiroz-junior Jôice Dias Corrêa Sílvia Maria Cordeiro Werneck Fabiana Simão Machado Thiago Mattar Cunha Gustavo Pompermaier Garlet Mauro Martins Teixeira Tarcília Aparecida da Silva |
| Abstract: | Aim: Leukotrienes (LTs) are pro-inflammatory lipid mediators formed by the enzyme 5-lipoxygenase (5-LO). The involvement of 5-LO metabolites in periodontal disease (PD) is not well defined. This study aimed to assess the role of 5-LO in experimental PD induced by Aggregatibacter actinomycetemcomitans (Aa). Material and methods: In vivo experiments were carried out using SV129 wild-type (WT) and 5-LO-deficient (5lo-/- ) mice inoculated with Aa. Osteoclasts were stimulated in vitro with AaLPS in the presence or not of selective inhibitors of the 5-LO pathway, or LTB4 or platelet-activating factor (PAF), as PAF has already been shown to increase osteoclast activity. Results: In 5lo-/- mice, there were no loss of alveolar bone and less TRAP-positive osteoclasts in periodontal tissues, after Aa inoculation, despite local production of TNF-α and IL-6. The differentiation and activity of osteoclasts stimulated with AaLPS were diminished in the presence of BLT1 antagonist or 5-LO inhibitor, but not in the presence of cysteinyl leukotriene receptor antagonist. The osteoclast differentiation induced by PAF was impaired by the BLT1 antagonism. Conclusion: In conclusion, LTB4 but not CysLTs is important for Aa-induced alveolar bone loss. Overall, LTB4 affects osteoclast differentiation and activity and is a key intermediate of PAF-induced osteoclastogenesis. |
| Subject: | Aggregatibacter actinomycetemcomitans Alveolar bone loss Leukotrienes Periodontal diseases Enzymes Proteins Osteoclasts Leukotriene B4 Osteogenesis Platelet activating factor |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE CLÍNICA ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICB - DEPARTAMENTO DE FARMACOLOGIA ICB - DEPARTAMENTO DE MICROBIOLOGIA ICB - DEPARTAMENTO DE MORFOLOGIA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1111/jcpe.12755 |
| URI: | http://hdl.handle.net/1843/83279 |
| Issue Date: | Aug-2017 |
| metadata.dc.url.externa: | https://onlinelibrary.wiley.com/doi/10.1111/jcpe.12755 |
| metadata.dc.relation.ispartof: | Journal of Clinical Periodontology |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.