Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/83391
Type: Artigo de Periódico
Title: Polymorphism of the beta-casein gene in holstein-zebu cows in the north of Minas Gerais, Brazil
Authors: Geziella Aurea Aparecida Damasceno Souza
Alessandra Rejane Ericsson de Oliveira
Anna Christina de Almeida
Mauro Aparecido de Sousa Xavier
Abstract: This study aimed to investigate A1 and A2 polymorphisms of the beta-casein gene in crossbred Holstein-Zebu (HZ) cows from four herds in Northern Minas Gerais, Brazil. Blood samples were collected from 112 randomly selected cows, of wich 66 were at ½ HZ, and 46 were at ¾ HZ. DNA extraction was conducted using the phenol-chloroform method, and polymorphisms were evaluated using the amplification refractory mutation system - polymerase chain reaction technique. The results demonstrated that 72/112 (64.3%) cows were heterozygous (A1A2), 33/112 (29.5%) were homozygous for A2A2, and 7/112 (6.2%) were A1A1 homozygous. The percentage of A2A2 cows ranged from 10.7 to 41.4% among the evaluated herds. The frequencies of the A1 and A2 alleles were 38.4 and 61.6%, respectively. The A2 allele was the most frequent in both breeds. The ½ HZ cows were 1.8 times more likely (Odds Ratio) to belong to the A1A1 genotype. The distribution of genotypes was in Hardy-Weinberg equilibrium in three of the herds; however, the total of samples and the stratified samples according to breeds were not in equilibrium considering α = 0.05.
Subject: Bovino de leite
Bovino de leite -- Desempenho
Reação em cadeia de polimerase
Leite -- Produção
language: eng
metadata.dc.publisher.country: Brasil
Publisher: Universidade Federal de Minas Gerais
Publisher Initials: UFMG
metadata.dc.publisher.department: ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
Rights: Acesso Aberto
metadata.dc.identifier.doi: https://doi.org/10.29367/tv7af190
URI: http://hdl.handle.net/1843/83391
Issue Date: 2024
metadata.dc.url.externa: https://revista.sodebras.com.br/index.php/revista/article/view/31
metadata.dc.relation.ispartof: Sodebras
Appears in Collections:Artigo de Periódico



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