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http://hdl.handle.net/1843/83745| Type: | Artigo de Periódico |
| Title: | Antileishmanial activity of fullerol and its liposomal formulation in experimental models of visceral leishmaniasis |
| Authors: | Guilherme Santos Ramos Frederic Jean Georges Frezard Virgínia Mendes Russo Vallejos Marina de Souza Ladeira Priscila Gomes dos Reis Daniel Menezes Souza Luiz Orlando Ladeira Mauricio Veloso Brant Pinheiro Maria Norma de Melo Ricardo Toshio Fujiwara |
| Abstract: | Visceral leishmaniasis (VL) is a systemic parasitic disease that leads to high rates of morbidity and mortality in humans worldwide. There is a great need to develop new drugs and novel strategies to make chemotherapy for this disease more efficacious and well tolerated. Recent reports on the immunomodulatory effects and the low toxicity of the spherical carbon nanostructure fullerol led us to investigate in vitro and in vivo antileishmanial activity in free and encapsulated forms in liposomes. When assayed against intramacrophagic Leishmania amastigotes, fullerol showed a dose-dependent reduction of the infection index with IC50 of 0.042 mg/mL. When given daily by i.p. route for 20 days (0.05 mg/kg/d) in a murine model of acute VL, fullerol promoted significant reduction in the liver parasite load. To improve the delivery of fullerol to the infection sites, liposomal formulations were prepared by the dehydration-rehydration method. When evaluated in the acute VL model, liposomal fullerol (Lip-Ful) formulations given i.p. at 0.05 and 0.2 mg/kg with 4-days intervals were more effective than the free form, with significant parasite reductions in both liver and spleen. Lip-Ful at 0.2 mg/kg promoted complete parasite elimination in the liver. The antileishmanial activity of Lip-Ful was further confirmed in a chronic model of VL. Lip-Ful was also found to induce secretion of pro-inflammatory TNF-α, IFN-γ and IL-1β cytokines. In conclusion, this work reports for the first time the antileishmanial activity of fullerol and introduces an innovative approach for treatment of VL based on the association of this nanostructure with liposomes. |
| Subject: | Fuleróis Lipossomos Leishmaniose |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ENG - DEPARTAMENTO DE ENGENHARIA QUÍMICA ICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA ICB - DEPARTAMENTO DE PARASITOLOGIA ICX - DEPARTAMENTO DE FÍSICA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.1016/j.biopha.2020.111120 |
| URI: | http://hdl.handle.net/1843/83745 |
| Issue Date: | 2021 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0753332220313135?via%3Dihub |
| metadata.dc.relation.ispartof: | Biomedicine & Pharmacotherapy |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Antileishmanial activity of fullerol and its liposomal.pdf | 391.87 kB | Adobe PDF | View/Open |
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