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http://hdl.handle.net/1843/83923| Tipo: | Artigo de Periódico |
| Título: | KRAS mutations drive adenomatoid odontogenic tumor |
| Autor(es): | Bruna Pizziolocoura Aline Carvalho Batista Marina Gonçalves Diniz Ricardo Santiago Gomez Sílvia Ferreira de Sousa Vanessa de Fátima Bernardes Josiane Alves França Helder Antonio Rebelo Pontes Danyel Elias da Cruz Perez Carolina Cavaliéri Gomes Ricardo Luiz Cavalcanti de Albuquerque Junior Manoela Domingues Martins |
| Resumo: | Objective: KRAS is the most frequently mutated onco gene in human neoplasms and we have previously reported KRAS p.G12V mutations in adenomatoid odontogenic tumors (AOT). We aimed to expand this cohort of samples and to test the association of KRAS mutations with clinical and histopatho logical parameters. A convenience sample of 30 AOT cases was included in the study. The hotpot KRAS p.G12V mutation was assessed by TaqMan allele-specific qPCR and codon 12 was direct sequenced. Clinical information obtained included patients age, tumor site, association of the lesion with impacted teeth and clinical tumor size. In addition, tumor capsule thickness was evaluated by morphometric analysis. Statistical analysis was car ried out to test the association of KRAS codon 12 mutations with clinico-pathological parameters. Findings: Molecular results confirmed KRAS p.G12V mutation in 14/23 cases, and p.G12R in 1/23. Eight cases were wild-type and samples from 7 cases failed amplification. Codon 12 mutations were not associated with any of the clinicopatho logical parameters tested (p>0.05). Conclusion: AOTshow high frequency of KRAS codon 12 mutations (15/23, 65%), which occur irrespectively of patients’ age, tumor location, association with impacted teeth, tumor clinical size or histopathological capsule thickness. |
| Assunto: | Association Mutation Codon Oncogenes Evaluation study |
| Idioma: | eng |
| País: | Brasil |
| Editor: | Universidade Federal de Minas Gerais |
| Sigla da Instituição: | UFMG |
| Departamento: | FAO - DEPARTAMENTO DE CLÍNICA ICB - DEPARTAMENTO DE PATOLOGIA |
| Tipo de Acesso: | Acesso Restrito |
| URI: | http://hdl.handle.net/1843/83923 |
| Data do documento: | Jul-2019 |
| metadata.dc.url.externa: | https://www.oooojournal.net/article/S2212-4403(19)30327-X/fulltext |
| metadata.dc.relation.ispartof: | Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology |
| Aparece nas coleções: | Artigo de Evento |
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