Atividade funcional de fagócitos do sangue periférico humano modulados por citocinas na presença de Giardia lamblia

Abstract

G. lamblia is the agent that causes the giardiasis, which is a disease with significant prevalence in the world, including developed countries, and the most common parasitic disease. Phagocytic cells and cytokines appear to be important in giardiasis, but the IFNluence of these cells and cytokines in protozoan IFNections is not totally elucidated. The aim of this study was to analyze the supernatant of cultures of MN cells with G. lamblia to determine: 1) the levels of the cytokines IFN-, TGF, IL-4 and IL-17; 2) the functional activity of MN cells after incubation with cytokines. Blood samples were collected from 60 volunteer donors healthy to obtain leukocytes. The levels of IFN- , TGF - , IL-4 and IL-17 were quantified in cell culture supernatants -trophozoite. The superoxide release, phagocytosis, microbicide activity, apoptosis and intracellular calcium release were analyzed during interactions of MN phagocytes and G. lamblia. It was observed that in culture supernatant of MN cells and G. lamblia were detected all the cytokines evaluated. The MN cells, regardless of kind of cytokines, in the presence of G. lamblia increased superoxide release. The phagocytosis, microbicide activity and apoptosis rates were higher when the MN phagocytes were treated by cytokines. The highest microbicidal and apoptosis rates were observed when the MN cells were treated by TGF-. The cytokines IFN- and IL-4 increased the release of intracellular calcium by MN phagocytes. The results suggest that cytokines play a beneficial role to the host by activation of MN cells for G. lamblia. They also suggest that during phagocytosis is occur death of G. lamblia the modulating the functional activity of phagocytes MN IFN-, TGF-, IL-4 and IL-17 appear to be essential in controlling the Giardiasis.