Vírus da artrite encefalite caprina: desenvolvimento e padronização de ensaios imunoenzimáticos (ELISA e Dot-Blot) e estudo epidemiológico no Estado do Ceará

Abstract

For a better knowledge and consequent implantation of the control measures for the arthritis encephalitis caprine in the state of Ceará an epidemic study was accomplished with emphasis in the flocks that have some genetic improvement and the native/SRD. A study of the health management used in the goat farms showing the more relevant sanitary problems was also done. It was verified that CAEV is not disseminated in the native/SRD flocks in the state of Ceará, however it is already present in several areas mainly in the milk production regions of the state. The bucks are more affected (P < 0,05) and can act, due to the practices commonly used, of changes or loan, an important paper in the transmission to the native/SRD flocks. It was also verified that the great majority producers of the caprine in the states of Ceará State explore their animals for meat/skin basically and that the sanitary handling of the animals of these farms in precarious, independent of the exploration type or system. Mortality of animals mainly youths, considered high committing the development of the activity. Another purpose of this work was to standardize an indirect ELISA using antigens of the whole virus for the diagnosis of CAE. Another thing was to develop and to validate a Dot-Blot test for the CAE serological diagnosis and to test the purification of the proteins of LVPR through the use of affinity chromatography. ELISA was shown to be more sensitive than IDGA being indicated for use in the control of LVPR. The Dot-Blot presents sensibility similar to indirect ELISA for the detection of antibodies against LVPR, because of the speed execution easiness, efficiency and little cost, it is an election test to be used in places of great movement and trade of small ruminant such as: exhibitions, fairs, shows, etc. The use of affinity chromatography in the purification of proteins of LVPR indicated it as viable alternative for production of purified antigen of LVPR.