Esplenectomia subtotal videolaparoscópica com preservação do polo inferior em ratos.

Abstract

Objective. Evaluate the exequibility and safety of subtotal splenectomy with preservation of the lower spleen pole (SS/LSPL), through laparoscopy and the viability of the remaining spleen. Method. 20 male Wistar rats, weighing between 365g and 474g (MA 417,92±36,15g) were subjected to (SS/LSPL) and distributed into two groups according with the time of withdrawal of the remaining for the purpose of the study: group I, the 10th day (n=10). After anesthesia and antissepsy of the abdominal cavity; three trocars were inserted into the abdominal cavity: the first one with 10mm, on the left iliac fossa under direct view of the abdominal cavity, followed by pneumoperitony. The second and third trocars of 5mm were inserted under laparoscopic view, one on the right sided subcostal region, and the other on the right iliac fossa. Right after this, the SS/LSPL was performed. The animals from group 1 and 2 were killed on the scheduled date. They were at the time weighed and subjected to exam of the abdominal cavity. The lower spleen pole and the upper portion was measured, weighed and sent to morphologic tests. Results. The technique was exequible in all cases. The average percentual weight of the lower pole evaluated in an indirect way on the 10th day was of 53,67% ± 11,59% and on the 80th day of 62,69% ± 6,89%, which implies increasing. The lower pole showed necrosis with abscess on one case on the group 2 and with normal aspect on the other cases. The microscopy showed compatible aspects with normality. Conclusion. The subtotal splenectomy with preservation of the lower pole by video laparoscopy was exequible on all the cases and was safety in 85% of them. The lower spleen pole proved to be viable in 94,1% of the animals