Prevalência de anticorpos contra o vírus da doença de Aujeszky em suínos no estado de Minas Gerais: comparação entre os métodos de soroneutralização de imunofluorescência indireta

Abstract

A study of 810 samples of pigs serum were analysed for antibodies agains Ajueszky Disease (AD), utilizing Serum-Neutralization (SN) in mouse and Indirect Fluorescent Antibody (IFI) technic. Of the total samples 556 were from a slaughterhouse and 254 from pig farms, of 31 counties in the state of Minas Gerais, Brazil. A total of 660 samples analyzed as a "pool" of five serums, by both methods (SN-IFI), thit not demonstrated any antibodies agains AD. From 235 serum-samples analyzed, only 5 (2,1%) were positive, with titles of 1:2 to 1:11 for SN and 1:2 to 1:8 for IFI. Hyperimmune serum from five pigs and seven rabbits previously immunized with inactivated virus (betapropiolactone) of a an experimental vaccine were utilized for standard SN and IFI technics. Neutralization of antibodies titer of rabbits serum, were determined by three differents temperatures and incubation times. At 37ºC and 90 minutes, titlle of 1/14.8DL50 were obtaimed, and this considered the best results, Hyperimmune serum of swine origen were title by SN and IFI, serum titer were positive by both methods. The higher titers were seen in SN from 1:30 to 1:110 and for IFI titers were 1:20 to 1:64.