Brucelose suína: prevalência em suínos abatidos em matadouros de Belo Horizonte e comparação da soro-aglutinação com outros métodos sorológicos

Abstract

To check the ocurrence of non-specific reactions in the slow agglutination test for the diagnostics of swine brucellosis and its prevalence in the State of Minas Gerais, 629 blood samples were collected from swine slaughtered in Belo Horizonte. First 62 sera were examined trough the following tests: slow agglutination test (SAT), indirect fluorescent antibody technique (IFAT), antiglobulin test (AGT), 2-mercaptoethanol test (2-ME). When SAT titer was 1:25 the other test, generally, were negative. However, titers equivalent to 1:50 showed regular agreement among the tests, with exception of ME. Thus the agreement found was CT 72,2%, AGT 66,6%, IFAT 50,0% and ME 27,8%. Good agreement was found when the titer was > 1:100, with the excpetion of the 2-ME test. Thus the agreement found was as follows: CT 100%, AGT 94,7%, IFAT 94,7% and 2-ME 63,1%. The AGT and IFAT showed, in general, higher titers in relation to the latter, equivalent or lower titers. The SAT and CT showed good agreement (96,1%) at a titer > 1:100 in 629 swine sera examined. The prevalence of brucellosis was 13,2% in the SAT and 17,3% in the CT. The prevalence found was 6 times greater in relation to official data, from the Federal Departament of Agriculture. There was no significant statistical difference in relation to sex in 69 samples examined.