Vírus da Doença de Aujeszky: diagnóstico, caracterização molecular e filodinâmica

Abstract

The suid herpesvirus 1 (SuHV-1) is the causative agent of pseudorabies or Aujeszky's disease, a contagious and epidemic disease that affects pigs in various stages of production. It is a notifiable disease by the World Organization for Animal Health, which can cause serious health barriers in international trade. The aim of this thesis was the development of molecular methods for direct application in the diagnosis and epidemiology of the etiological agent of pseudorabies. The molecular diagnostic methods developed for the detection of SuHV-1 had a high correlation with viral isolation and neutralization. Besides these, other techniques were developed to detect SuHV-1 and other agents causing respiratory diseases in pigs by multiplex PCR in real time. In addition to high sensitivity and specificity, these methods have been considered practical and quick to detect SuHV-1 and related agents. Since certain methods for diagnostic agent for pseudorabies have been developed methods for the molecular characterization of the virus. The first step was to make it practical and fast genotyping by restriction enzyme SuHV-1 then for further study by molecular sequence of the gene ul44, US9, US8, U14, ul49.5. The data generated in these analyzes allowed to define phylogenetic groups of the virus distributed around the world and to differentiate the viruses isolated from domescit and wild swine. The studies of this thesis developed new rapid, sensitive and specific diagnostics for detection of SuHV-1 which can be used in conjunction with molecular characterization techniques which will aid in the understanding of the epidemiology of pseudorabies.