Aplicação da técnica de interferência por RNA para inibição de tumor, e microRNAs com função supressora de tumor

Abstract

The oncogenesis represents an important public health problem, and therefore gene therapy has evaluated the technique of RNA interference, considered a powerful technique for the study of gene function and development of gene-specific therapy. Mediated RNA interference is a phenomenon of post transcriptional regulation that occurs naturally ineukaryotic organisms and plays the role of elimination of messenger RNAs, working mainly inthe body's defense against molecular parasites such as transposons and viruses (Zerbini et al., 2006). After this discovery, gene therapy has invested in the study of microRNAs withtumor suppressor function and also of interfering RNA molecules which, when introducedinto cells, it induces downregulation of genes (Ring et al. 2002). The tool presented in this work, therefore, was the construction of viral vectors to multiply and specifically lyse tumor cells. As the brand of human tumors is the loss of function of p53 tumor suppressor protein,the aim is retune the adenoviral tropism via a viral RNA interference in the expression of ap53-dependent promoter. This mechanism of RNA interference suppression promoted adenoviral gene and its consequent multiplication in normal cells, since these cells normally express p53. However, there was tumor by inhibiting viral multiplication in cellsdysfunctional p53 (Gürlevik et al. 2009).