Avaliação da influência do background genético no reparo de lesão interna e na reação tipo corpo estranho em camundongos diabéticos

Abstract

Diabetes reduces quality of life by causing complications such as neuropathies, limb amputations, retinopathies, and a high risk of cardiovascular disease. Faced with this reality, a better understanding of the pathophysiological mechanisms of diabetes and its complications becomes essential. Evidence in the literature indicates that exogenous factors such as environmental factors and endogenous factors such as the genetic background, determine the manifestation of several pathological processes. Several genetic factors have been associated with the development of diabetes and its complications. However, to what extent this susceptibility influences internal healing involving inflammatory angiogenesis and the foreign body response to a biomaterial implant (glucose sensors, orthopedic implants, catheters, vascular grafts, etc.) has not been fully investigated. In this work we studied the internal healing and foreign body type reaction in the fibrovascular tissue induced by implants of a synthetic polyether polyurethane matrix in Swiss, C57BL/6 and Balb/c mice induced by streptozotocin diabetes. Hyperglycemic levels (mg / dl) following diabetogenic treatment were 455.0 ± 15 in Swiss mice, 393.0 ± 22 in C57BL/6 and 190.0 ± 10 in Balb/c. Inflammation, angiogenesis, and cytokine production (key components) in the internal injury repair process and foreign body type response in both normoglycemic and hyperglycemic animals occurred in a specific lineage manner. Angiogenesis in implants of non-diabetic Swiss mice was higher than in implants of other strains. In the hyperglycemic environment, almost all inflammatory markers increased in implants of Swiss and C57BL/6 diabetic mice. All foreign body type reaction characteristics were higher in Swiss and C57BL/6 mice implants compared to those in Balb/c diabetic implants. This study demonstrates the main contribution of the genetic background in the inflammatory angiogenesis components of the internal lesion and the foreign body type response in both normoglycemic and hyperglycemic animals and in the intensity of the biomaterial response. This variability may be relevant when considering the animal model of diabetes and therapeutic approaches in internal healing and in therapeutic approaches that use implantable devices in diabetics.