Estresse oxidativo: avaliação in vitro da capacidade moduladora do Resveratrol em células da linhagem Neuro 2-A

Abstract

Aging is a natural and physiological process that progressively compromises the individual's physical and cognitive status, increasing the risk of morbidities such as: cancer, cardiovascular diseases and mortality. It is associated with a high occurrence of metabolic alterations and disorders. "Oxidative Stress" stands out among the main elements involved in these strident modifications. In addition, cerebral aging is a phenomenon, too, intricate and genuine, and is determined exclusively by oxidative stress, accumulation of oxidatively damaged macromolecules and changes in the structure and function of neurons, which further increase the risk factor for diseases. Too much data supporting the important role of oxidative stress in the aging process and neurodegenerative diseases supports the beneficial effect of antioxidants as adjuvant therapy. Antioxidants are compounds capable of inhibiting or retarding the oxidation of biomolecules. They can be enzymatic or non-enzymatic, being produced endogenously or not. Among the non-enzymatic, we have the polyphenols, distributed in fruits, vegetables, beverages and cereals. As is the case with Resveratrol. Our study aimed to verify the possible Neuroprotective and / or Modulator effect of resveratrol in N2-A cells (albino mouse neuroblastoma) previously or late in an "oxidative stress environment" caused by exposure to peroxide hydrogen. The dose of resveratrol was standardized at 5M; and hydrogen peroxide in 10.5% v / v, both through Concentration-Response curve. For the evaluation of Oxidative and Reducing Compartments in N2-A Cells, we performed the following tests: fluorescence microscopy with the use of Carboxy-H2DCFDA, production of nitric oxide and peroxynitrite, cellular reducing capacity by MTT. We also verified the activity of catalase and superoxide dismutase in this cell line. We analyzed the involvement of AMPk and SIRT1 signaling pathways on RSV activity in N2-A cells. Finally, we proceeded with the evaluation of the inflammatory profile in these neural cells through the activity of IL-6, TNF- and IL-10. We observed in this study that RSV 5M in N2A cells exerts antioxidant, anti-inflammatory and neuro-protective power. And the same occurs mostly in the form of Prevention.