Constituintes mitocondriais circulantes após transplante hepático e a sua correlação com marcadores de necrose celular

Abstract

The natural immune system is essential to bodys defense against viruses, bacteria and protozoa. It is present in evolutionary chain since annelids. Macrophages and neutrophils and other cells, not direct related to the immune system, have receptors on their membrane and cytoplasm that recognize viruses and bacteria. Natural immune system receptors activation is critical for protection and cleaning of tissue, fundamental step towards healing. Ischemia and reperfusion injury and cell death occur in liver transplantation. The constituents of hepatocytes spill out of the cell triggering the immune response. This immune response has usually small impact but in case of excessive stimulation may cause more inflammation and necrosis stimulation, with serious consequences as no primary graft functions, early dysfunction of the liver and systemic inflammatory response with multiple organ failure. Among these molecular structures are the mitochondrial structures. The objective of this study was evaluate the presence of circulating mitochondrial constituents during perioperative period in liver transplantation and correlates them with aminotransferases and the ischemia time. . The following mitochondrial acids deoxyribonucleic were measured: DNA nicotinamide adenine dinucleotide (NADH), DNA cytochrome B e DNA cytochrome C oxidases in blood samples of patients undergoing hepatic transplantation from cadaver donor collected immediately before portal vein clamping and eight hours after graft reperfusion. It was found a significant increase of the mitochondrial constituents after the transplant procedure and its association with the raise of hepatic enzymes (aspartatoaminotransferase - AST e alaninoaminotransferase - ALT). These findings may contribute for a better understanding of the phenomenon: natural immune response stimulated by ischemia and reperfusion injury in the hepatic transplantation.