Avaliação da caracterização de micropartículas após estimulo de células mononucleares de sangue periférico por microparticulas em pacientes com Lúpus Eritematoso Sistêmico em comparação com pacientes com outras doenças autoimunes.

Abstract

Systemic Lupus Erythematosus (SLE) is a chronic and systemic autoimmune disease. Microparticles (MPs) can lead to inflammation and autoimmunity by multiple mechanisms and display the cell surface proteins of the parent cell that can be detected by flow cytometry and fluorescence microscopy allowing identification of their origin. Although clinical and laboratory criteria are currently used to monitor therapeutic effects, there is still no marker of remission of disease or activity. Increased levels of cell-derived MPs and the parent cell may suggest a significant influence on the prognosis of the disease. This study aims to characterize the MPs of patients with SLE, originated from cells CD4+, CD8+, CD14+, CD20+, CD42a+, CD54+ e CD61+ gifts in the plasma of patients with low and moderate/high activity and IL-10 and IFN- production by peripheral blood mononuclear cells (PBMC) after stimulation with autologous MPs from these patients through specific antibody labeling by flow cytometry (CF), aiming a better understanding of their role in the modulation of the immune response in SLE. The study was conducted in patients, fifteen female with SLE diagnosed by the American College of Rheumatology (ACR), seven with low activity SLEDAI-2k4 (LESABA), eight with moderate / high activity SLEDAI-2k>4 (LESAMA), and 2 two control groups, 5 women with other autoimmune diseases (ODA) and 5 healthy women-volunteers (CT). In the CT group of MPs derived from CD4 + and CD14 + cells were significantly different when compared with LESABA, LESAMA and ODA groups. There was no significant difference in the levels of MPs among the different groups of patients. There was no significant difference in the level of CD8 +, CD42a +, CD54 + and CD61 + MPs among the different groups in study.However, in the ODA group the IFN- production by CD8 + cells was higher than CT and LESAMA groups, but there was no difference in IFN- production by CD4 + cells. No significant difference was observed in CD4 +, CD8 +, CD14 + and CD19 + cells of PBMC after stimulation with MPs as well as in the IL-10 production by CD4 + and CD8 +.This study has a significant importance because now there are no specific markers that can be used in the diagnosis and prognosis of lupus.