Comparação do desempenho de meios de cultura Lowenstein-Jensenproduzidos com diferentes tipos de ovos para realização do teste desensibilidade aos fármacos no tratamento da tuberculose

Abstract

Tuberculosis is an infectious disease transmitted from person to person, considered a serious public health problem worldwide. It is caused by the bacterium Mycobacterium tuberculosis, affecting mainly the lungs. The definite diagnostics of TB is make by identification of Mycobacteria present in a biological sample, by the culture exam, considered the gold-standard, because provides high sensibility andspecificity for detection of pulmonary and extrapulmonary TB. It is standardized to use in the formulation of the culture medium, eggs from chicken that did not receive antibiotics in the feed, with a posture of no more than seven days, and evidence by certification and traceability of the batches. Currently is used in Funed, for research, the non-embryonated egg, however there are other types of eggs, such as organic and rustic which are also submitted to the required requirements. The objective ofthis work was compare the performance of the Lowenstein-Jensen solid culture medium, using in its formulation three different types of eggs: the rustic egg, the nonembryonated egg, intended for research, and the organic egg. Comparatives tests of the medium performance were carried out with these eggs, ensuring, in this way, that an exchange would not influence in the performance of the culture medium. Beingcarried out performance analysis of visual control , sterility, pH and Sensibility test by the method of Indirect Proportions to the drugs Streptomycin, Isoniazid, Rifampin and Ethambutol with inoculation of strains with known and standardized sensibility and resistance profiles. After the analysis of all the carried out tests, was verified that thethree batches presented satisfactory results in the analysis of visual control, pH range, sterility test, and sensibility test, in relation to the established criteria, besides showing identical performance in the Test of Sensibility, however the ease of warranty of certification and traceability is greater for the organic egg compared to the rustic egg. Thus, it is possible to conclude that the LJ culture medium produced with the organic egg it is submitted to the quality criteria required, traceability of the entire batch, besides making the commercialization viable because it decreases the cost with the egg for production in 95%, since the annual cost could pass average R$102.000,00 .00 to R$ 5.000,00.