Avaliação da atividade amidásica da calicreína tecidual humana na urina de pacientes com diabetes mellitus gestacional e de mulheres sadias gestantes e não gestantes

Abstract

Diabetes mellitus (DM) is a group of metabolic disorders on carbohydrate metabolism in which glucose, being underutilized, produces hyperglycemia. Gestational diabetes mellitus (GDM) is a carbohydrate intolerance of variability severity with onset or first recognition during pregnancy. It can persist or disappear after the childbirth. The role of urinary human tissue kallikrein (hK1) in DM is notclear yet. Previous studies describe the involvement of urinary hK1 in the type 1 DM pathogenesis. The aim of the present study was to evaluate the role of urinary hK1 in GDM patients. Thirty GDM patients were selected. Thirty healthy pregnant and thirty healthy non-pregnant women, were used as controls. A random sample urine collection was used. Protein was determined with Coomassie Brilliant Blue G- 250. Creatinine was determined by Jaffes method. hK1 amidase activity was estimated with D-Val-Leu-Arg-Nan substrate and expressed in ìM . min1 . mg1creatinine to correct for differences in urine flow. Protein/creatinine ratio (PCR) was calculated. hK1 amidase activity was significantly higher in the urine of GDM patients than in healthy pregnant and in healthy non-pregnant women. On the other hand, hK1 amidase activity was not statistically different between healthy pregnant and healthy non-pregnant women. Creatinine excretion was not statistically different between GDM patients and the other groups of women. On the other hand, creatinine excretion was statistically higher in the healthy nonpregnant than in healthy pregnant women. PCR was higher in DMG patients thanin the other groups of women. Its given credit that the hyperglycemic status cause the increase of hK1 amidase activity in GDM patients.