Avaliação da expressão apical de fatores pró-angiogênicos e citocinas em reposta à infecção endodôntica

Abstract

The progression and development of pulpal and periapical pathologies are closely related to the presence of microorganisms and their by-products in the infected root canal system (RCS), which induces a defense response adjacent to the root apex. Angiogenesis has been identified as an essential factor in the pathogenesis of chronic periapical alterations, being related to its establishment and maintenance, being a constant source of cytokines, chemokines and proteases. Angiogenesis is also related to the tissue repair that follows the resolution of the periradicular alterations after the implementation of endodontic therapy. In this study, was evaluated the expression of pro-angiogenic factors and correlated cytokines in samples collected from patients (n = 20) on teeth with Chronic Apical Periodontitis immediately after RCS instrumentation and 7 days after disinfection procedures. Samples were analyzed by real-time polymerase chain reaction (RT-PCR). The gene expression of pro-angiogenic factors and cytokines Angiopoetin-1 (AGT1), Vascular endothelial growth factor-A (VEGF-A), Basic fibroblast growth factor (FGF-), Monocyte Chemoattractant Protein-1 (CCL2/MCP-1), Macrophage inflammatory protein-1 (CCL4), C-X-C chemokine receptor motif 4 (CXCR4), C-C chemokine receptor motif 6 (CCR6), TNF-, IFN-, IL-17, IL-10, IL-6, RANK-L and MMP-9. The mRNA expression of the mediators evaluated revealed a significant increase in levels of AGT1, CCL2/MCP-1, CCL4, CCR6, TNF-, IFN-, IL-10, RANK-L and MMP-9 on day 7 when compared to day 0 (P <0.05). As for VEGF-A, FGF-, CXCR4, IL-17 and IL-6 their mRNA expressions was similar at both observed times (P> 0.05). In conclusion, after cleaning and shaping procedures of the RCS, there was an increase in mRNA expression levels of important mediators involved in angioproliferative and osteogenic phenomenon.