Perfil bioquímico, microbiológico e celular do leite de vacas girolandas do parto aos 180 dias de lactação

Abstract

This study evaluated the incidence of pathogens, as well as Somatic Cells Count, Bacterial Quantification, biochemical evaluation and differential cell from 225 milk samples from February to November 2012. Twenty five crossbred cows, pluriparous, from a commercial farm located in the district of Ibitira, Martinho Campos, Minas Gerais were evaluated. To collect, pre-dipping routinely made by the farm, then cleaning the ceilings with gauze soaked in 70% alcohol was performed. After this procedure the disposal of the first three streams of milk was performed and conducted to store. The collections were made on the exact day of delivery and colostrum, 5, 10, 30, 60, 90, 120, 150 and 180 days thereafter. After milk collection, the slides were prepared and stained for Bacterial Quantification, estimated somatic cell and differential cell count. The remaining material was used for bacterial isolation and biochemical evaluation. In somatic cell count, decreased between the third and fourth months of lactation and increased for the middle third lactation was observed. About 48% of the animals evaluated at all times had Somatic Cells Count above 200,000 cells/ml of milk. For the presence of pathogens, of 25 cows, 18 (72%) were positive for Staphylococcus aureus in early lactation and only this pathogen was present until the end of the experiment. The most prevalent pathogens in the course of this study were Staphylococcus aureus, Streptococcus uberis and Bacillus spp. In biochemical evaluation in automatic device, when there was an increased concentration of somatic cells, there was an increase in the concentration of chloride, calcium, magnesium, alkaline phosphatase and gamma- glutamyl transferase in milk. The differential cell performed by optical microscopy, was increased when the concentration of somatic cells, it increased the concentration of neutrophils, lymphocytes, eosinophils, macrophages, epithelial cells and basophils.