Detecção e identificação de campylobacter spp. em carcaças de frango de corte produzidas no estado de Minas Gerais

Abstract

The food-transmitted illness are considered an immense issue to the world general health and between the microorganisms who can be conveyed that way, the bacterium Campylobacter spp. standouts, it frequently occurs on poultry carcasses. Striving to detect, depict and evaluate the occurrence of campylobacter spp. in poultry carcasses, frozen and chilled carcasses, collected from the state of Minas Gerais, were analyzed using three distinct methodologies (culture, real time PCR and real time PCR associated with the use of propidium monoazide). A hundred samples of poultry carcasses (40 chilled and 60 frozen) were collected from sixteen poultrys slaughterhouse along the state and each one were submitted to tests using the three methodologies. The culture method showed positive results to the presence of Campylobacter spp. in 28 (28%) carcasses, and from these 14 carcasses were frozen and 14 chilled. The PCR methodology, 84 (84%) samples were positive to the microorganism, and from these 47 were frozen and 37 chilled. From the samples analyzed using the real time PCR with the use of PMA, (DNA intercalate capable of penetrate in non-viable cells, preventing that its DNA be amplified by the real time PCR) eight frozen (31%) and 18 chilled (69%) returned positive results to the presence of the bacterium. The culture method also returned positive results to the presence of Campylobacter jejuni from 11 (39%) samples, Campylobacter coli from 4 (15%) samples and Campylobacter lari from 2 (7%) samples. The results also showed that less frozen carcasses, in comparison to those who were chilled, presented the microorganism. We can conclude that the occurrence and viability of the microorganism Campylobacter spp. in poultry carcasses from the state of Minas Gerais is high and that the culture and real time PCR with the use of PMA are efficient in detecting the presence of this pathogen.