Pesquisa de resíduos de anticoccidianos por LC/MS-MS em frangos de corte submetidos a tratamento farmacológico

Abstract

In order to evaluate the presence and levels of anticoccidial residue in broiler chickens muscle, by high-performance liquid chromatography-tandem mass spectrometry (HPLC / MS-MS) were used 720 broiler chickens from Cobb line, reared by 41 days. The treatments, defined according to the anticoccidial provided by ration to poultry, were as follows: A- control (no anticoccidial throughout the breeding period); B- nicarbazin (with anticoccidial from the first to the 21th day of rearing); C- nicarbazin + narasin (with anticoccidial from the first to the 28th day of rearing); D salinomycin (with anticoccidial from the first to the 34th day of rearing); E semduramicin (with anticoccidial from the first to the 34th day of rearing) and; F- monensin (with anticoccidial from the first to the 34th day of rearing). To collect thigh and breast muscle samples and evaluation of drug residues used were slaughtered six birds per treatment weekly during the feeding period with anticoccidial and for six days after the discontinuation of the drug. Nicarbazin residues (dinitrocarbalinida - DNC) were observed in both treatment where it was used until the sixth day of the withdrawal period and until the fourth day of withdrawal period, the DNC values were above the maximum residue limit (MRL ) acceptable by global regulatory agencies (200 g.Kg-1). Semduramicin residues above the MRL (50 g.Kg-1) were only observed during feeding period with the drug and narasin and monesin residues did not exceed the MRL values (15 g.Kg-1 e 10 g.Kg-1, respectively) neither the feeding period with the drugs. The salinomycin showed no possible residues to be detected by the method used throughout the treatment period.