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http://hdl.handle.net/1843/UCSD-854JCH| Type: | Dissertação de Mestrado |
| Title: | Clonagem e caracterização molecular de cisteíno proteases de Carica candamarcensis |
| Authors: | Caroline Furtado Junqueira |
| First Advisor: | Carlos Edmundo Salas Bravo |
| First Referee: | Alfredo Miranda de Goes |
| Second Referee: | Almir de Sousa Martins |
| Abstract: | As Caricaceaes são uma importante fonte de enzimas proteolíticas pertencentes ao grupo das cisteíno proteases. Estas enzimas são economicamente relevantes por possuírem diversas aplicações industriais e terapêuticas. Cisteíno proteases de Carica candamarcensis vêm sendo estudadas pelo nosso grupo por possuírem de cinco a oito vezes mais atividade proteolítica do que as enzimas de C. papaya. Quinze destas proteínas já foram caracterizadas no nível protéico, porém, nunca foram clonadas a partir de mRNA. Usando uma preparação de mRNA da folha da planta, moléculas de cDNA foram sintetizadas por RT-PCR e clonadas em plasmídios de sequenciamento e de expressão. Aqui são relatados os resultados de clonagem e caracterização mlecular de duas cisteíno proteases desta espécie. |
| Abstract: | Caricaceaes are important source of proteolytic enzymes belonging to the group of cysteine proteinases. These enzymesare economically relevant to several industrial and therapeutic applications. Cysteine proteinases from Carica candamarcensis are being studied by our group for their high proteolytic activity stimated between five to eight fold higher than enzymes from C. papaya. Although four of these enzymes were previously characterized at the proteolytic level, however, none of them were cloned from a mRNA. Using an mRNA plant leaf preparation, cDNA molecules were synthesized by RT-PCR and cloned into sequence and expression vectors. Here are related the results of a cloning and molecular characterization regarding to two cysteine proteinases from this species. |
| Subject: | Bioquímica Cisteíno proteinase |
| language: | Português |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| Rights: | Acesso Aberto |
| URI: | http://hdl.handle.net/1843/UCSD-854JCH |
| Issue Date: | 27-Feb-2007 |
| Appears in Collections: | Dissertações de Mestrado |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| resumo_mestrado_caroline_f.__junqueira.pdf | 80.94 kB | Adobe PDF | View/Open |
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