ST2 regulates bone loss in a site-dependent and estrogen-dependent manner

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dc.creatorSoraia Macari
dc.creatorGustavo Pompermaier Garlet
dc.creatorMauro Martins Teixeira
dc.creatorTarcília Aparecida da Silva
dc.creatorMila Fernandes Moreira Madeira
dc.creatorIzabella Lucas de Abreu Lima
dc.creatorThaís dos Santos Fontes Pereira
dc.creatorGeorge J Dias
dc.creatorJoni Augusto Cirelli
dc.creatorRafael Scaf de Molon
dc.creatorSandra Yasuyo Fukada
dc.creatorRaphael Escorsim Szawka
dc.date.accessioned2025-04-22T22:41:47Z
dc.date.accessioned2025-09-09T00:06:42Z
dc.date.available2025-04-22T22:41:47Z
dc.date.issued2018-10
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1002/jcb.27080
dc.identifier.issn1097-4644
dc.identifier.urihttps://hdl.handle.net/1843/81768
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Cellular Biochemistry
dc.rightsAcesso Restrito
dc.subjectAlveolar bone loss
dc.subjectBiomarkers
dc.subjectEstrogens
dc.subjectInterleukin-33
dc.subjectMaxilla
dc.subjectOsteoporosis
dc.subjectOvariectomy
dc.subjectRNA, messenger
dc.subjectBone remodeling
dc.subject.otherST2
dc.subject.otherAlveolar bone loss
dc.subject.otherEstrogens
dc.subject.otherInterleukin-33
dc.subject.otherMaxilla
dc.subject.otherOsteoporosis
dc.subject.otherSite specific
dc.titleST2 regulates bone loss in a site-dependent and estrogen-dependent manner
dc.typeArtigo de periódico
local.citation.epage8521
local.citation.issue10
local.citation.spage8511
local.citation.volume119
local.description.resumoInterleukin-33 (IL-33) and its receptor, ST2, are implicated in bone remodeling. The lack of estrogen after menopause results in an accelerated bone loss. Here we investigated the role of ST2 in the bone loss induced by estrogen deficiency. ST2-deficient mice (ST2-/- ) and their littermates (wildtype [WT]) were ovariectomized (OVX), while ovary-intact mice were used as controls. Bone sites were analyzed by microcomputed tomography, histomorphometry, and quantitative real-time polymerase chain reaction (qPCR). Deletion of IL-33 or ST2 resulted in a similar bone loss in the femur and maxilla. Ovariectomy in WT mice caused bone loss in the same areas. The lack of ST2 in OVX mice did not alter bone remodeling in the femur but prevented bone loss in the maxilla. Consistently, ovariectomy increased the IL-33 messenger RNA (mRNA) levels in the maxilla but not in the femur. Under mechanical stimulation, ovariectomy and ST2 deletion independently increased bone remodeling induced by orthodontic tooth movement, which was also associated with a greater number of osteoclasts and a reduced number of osteoblasts in the maxillary bone. ST2-/- OVX mice, however, displayed twice as many osteoblasts as that of WT OVX mice. Ovariectomy and ST2 deletion differently altered the cytokine mRNA levels in the maxilla. Remarkably, interleukin-10 expression was decreased in both WT OVX and ST2-/- mice, and this reduction was completely restored in ST2-/- OVX mice. The results demonstrate that estrogen and IL33/ST2 independently protect against bone loss. However, the ovariectomy-induced bone loss is IL-33/ST2-dependent in the maxilla but not in the femur, indicating a bimodal and site-specific role of ST2 in bone remodeling.
local.identifier.orcidhttps://orcid.org/0000-0001-7643-6589
local.identifier.orcidhttps://orcid.org/0000-0002-5071-8382
local.identifier.orcidhttps://orcid.org/0000-0002-6944-3008
local.identifier.orcidhttps://orcid.org/0000-0001-9623-7835
local.identifier.orcidhttps://orcid.org/0000-0002-1770-9978
local.identifier.orcidhttps://orcid.org/0000-0002-7730-1587
local.identifier.orcidhttps://orcid.org/0000-0003-3197-0061
local.identifier.orcidhttps://orcid.org/0000-0002-7082-9290
local.identifier.orcidhttps://orcid.org/0000-0003-1110-6233
local.identifier.orcidhttps://orcid.org/0000-0002-6589-4784
local.identifier.orcidhttps://orcid.org/0000-0002-2639-3469
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.departmentICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1002/jcb.27080

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