Pre-exposure with extracellular vesicles from Aspergillus fumigatus attenuates inflammatory response and enhances fungal clearance in a murine model pulmonary aspergillosis

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dc.creatorJéssica Amanda Marques Souza
dc.creatorIsabella Luísa da Silva Gurgel
dc.creatorNathália Luísa Sousa de Oliveira Malacco
dc.creatorFlávia Rayssa Braga Martins
dc.creatorCelso Martins Queiroz Junior
dc.creatorMauro Martins Teixeira
dc.creatorFrederico Marianetti Soriani
dc.date.accessioned2023-10-30T21:36:33Z
dc.date.accessioned2025-09-09T01:06:17Z
dc.date.available2023-10-30T21:36:33Z
dc.date.issued2022-06-01
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3389/fcimb.2022.898619
dc.identifier.issn22352988
dc.identifier.urihttps://hdl.handle.net/1843/60294
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofFrontiers in Cellular and Infection Microbiology
dc.rightsAcesso Aberto
dc.subjectVesículas extracelulares
dc.subjectAspergillus fumigatus
dc.subjectImunização
dc.subjectInterações hospedeiro-patógeno
dc.subject.otherExtracellular vesicles
dc.subject.otherFilamentous fungus
dc.subject.otherAspergillus fumigatus
dc.subject.otherImmunization
dc.subject.otherHost-pathogen interactions
dc.titlePre-exposure with extracellular vesicles from Aspergillus fumigatus attenuates inflammatory response and enhances fungal clearance in a murine model pulmonary aspergillosis
dc.typeArtigo de periódico
local.citation.epage13
local.citation.spage1
local.citation.volume12
local.description.resumoAspergillus fumigatus is a ubiquitous and saprophytic filamentous fungus and the main etiologic agent of aspergillosis. Infections caused by A. fumigatus culminate in a strong inflammatory response that can evolve into respiratory failure and may be lethal in immunocompromised individuals. In the last decades, it has been demonstrated that extracellular vesicles (EVs) elicit a notable biological response in immune cells. EVs carry a variety of biomolecules, therefore are considered potential antigen delivery vehicles. The role of EVs as a strategy for modulating an effective response against infections caused by A. fumigatus remains unexplored. Here we investigate the use of EVs derived from A. fumigatus as an immunization tool to induce a more robust immune response to A. fumigatus pulmonary infection. In order to investigate that, male C57BL/6 mice were immunized with two doses of EVs and infected with A. fumigatus. Pre-exposure of mice to EVs was able to induce the production of specific IgG serum for fungal antigens. Besides that, the immunization with EVs reduced the neutrophilic infiltrate into the alveoli, as well as the extravasation of total proteins and the production of proinflammatory mediators IL-1b, IL-6, and CXCL-1. In addition, immunization prevented extensive lung tissue damage and also improved phagocytosis and fungus clearance. Noteworthy, immunization with EVs, associated with subclinical doses of Amphotericin B (AmB) treatment, rescued 50% of mice infected with A. fumigatus from lethal fungal pneumonia. Therefore, the present study shows a new role for A. fumigatus EVs as host inflammatory response modulators, suggesting their use as immunizing agents.
local.identifier.orcidhttps://orcid.org/0000-0003-4720-6746
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE FARMACOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.frontiersin.org/articles/10.3389/fcimb.2022.898619/full

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