Campomanesia guazumifolia infusion decreases human platelet aggregation by reducing cyclooxygenase 1 activity and its underlying mechanisms

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dc.creatorCaroline Honaiser Lescano
dc.creatorIvan Pires de Oliveira
dc.creatorFernando Freitas de Lima
dc.creatorTaline Baganha Stefanello Catelan
dc.creatorVictor Hugo Dantas Guimarães
dc.creatorUlisses Alves Pereira
dc.creatorCharles Martins Aguilar
dc.creatorCláudia Andrea Lima Cardoso
dc.creatorSérgio Henrique Sousa Santos
dc.creatorAlexandre Borges
dc.date.accessioned2024-09-24T14:22:27Z
dc.date.accessioned2025-09-09T00:19:32Z
dc.date.available2024-09-24T14:22:27Z
dc.date.issued2023
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1016/j.fbio.2023.102955
dc.identifier.issn2212-4292
dc.identifier.urihttps://hdl.handle.net/1843/76840
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofFood Bioscience
dc.rightsAcesso Restrito
dc.subjectGuabiroba
dc.subjectPlantas dos cerrados
dc.subjectFlavonoides
dc.subjectCálcio
dc.subjectTromboxanas
dc.subject.otherGuabiroba
dc.subject.otherPlantas dos cerrados
dc.subject.otherFlavonoides
dc.subject.otherCálcio
dc.subject.otherTromboxanas
dc.titleCampomanesia guazumifolia infusion decreases human platelet aggregation by reducing cyclooxygenase 1 activity and its underlying mechanisms
dc.title.alternativeA infusão de Campomanesia guazumifolia diminui a agregação plaquetária humana reduzindo a atividade da ciclooxigenase 1 e seus mecanismos subjacentes
dc.typeArtigo de periódico
local.citation.epage12
local.citation.spage1
local.citation.volume55
local.description.resumoCampomanesia guazumifolia is a species from the Brazilian Cerrado, and its leaves are used by traditional medicine in the preparation of infusion. Its popular uses include antidiarrheal, hypocholesterolemic, and anti-inflammatory purposes. Here we studied the leaf infusion of Campomanesia guazumifolia (LICG) on platelet activity, investigating its role in the cyclooxygenase (COX) pathway. For this, the phytochemical composition, in vivo toxicity (using zebrafish) were evaluated and their effects were investigated on platelet reactivity using different agonists. To elucidate the possible mechanisms involved, we studied the impact of LICG on calcium mobilization, cyclic nucleotides and thromboxane levels, PKCβ2 phosphorylation, and COX inhibition. Furthermore, in silico studies showed the interactions of COX-1 with the compounds identified in LICG by molecular modeling. We describe for the first time that LICG acts by inhibiting platelet aggregation evoked by different physiological agonists, which results in a drastic reduction in intracellular calcium discharge without the involvement of cyclic nucleotide pathways. Furthermore, we showed that LICG appears to inhibit cyclooxygenase 1, reducing thromboxane A2 levels and PKCβ2 phosphorylation in human platelets, reinforced by similar interactions compared to a reference inhibitor at the catalytic site. This study demonstrates the antiplatelet effects of LICG via COX-1 and suggests that these agents may be therapeutic for preventing platelet-associated cardiovascular disease.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S2212429223006065

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