Using Next-Generation Sequencing (NGS) to uncover diversity of wood-decaying fungi in neotropical atlantic forests

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dc.creatorAline Bruna Martins Vaz
dc.creatorPaula Luize Camargos Fonseca
dc.creatorLaura Rabelo Leite
dc.creatorFernanda Badotti
dc.creatorAnna Cristina de Matos Salim
dc.creatorFlávio Marcos Gomes Araújo
dc.creatorSara Cuadros Orellana
dc.creatorÂngelo Amâncio Duarte
dc.creatorCarlos Augusto Rosa
dc.creatorGuilherme Oliveira
dc.creatorAristóteles Góes Neto
dc.date.accessioned2023-06-19T20:05:04Z
dc.date.accessioned2025-09-09T00:11:25Z
dc.date.available2023-06-19T20:05:04Z
dc.date.issued2017
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipOutra Agência
dc.format.mimetypepdf
dc.identifier.doihttp://dx.doi.org/10.11646/phytotaxa.295.1.1
dc.identifier.issn1179-3163
dc.identifier.urihttps://hdl.handle.net/1843/55123
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPhytotaxa
dc.rightsAcesso Aberto
dc.subjectMetagenômica
dc.subjectMadeira-microbiologia
dc.subjectFloresta Úmida
dc.subjectFlorestas tropicais
dc.subject.otherFungal metabarcoding
dc.subject.otherRainforest
dc.subject.otherSeasonal tropical forest
dc.subject.otherLignocellulosic residues
dc.titleUsing Next-Generation Sequencing (NGS) to uncover diversity of wood-decaying fungi in neotropical atlantic forests
dc.title.alternativeUsando Sequenciamento de Nova Geração (NGS) para descobrir a diversidade de fungos decompositores de madeira em florestas atlânticas neotropicais
dc.typeArtigo de periódico
local.citation.issue1
local.citation.volume295
local.description.resumoA targeted amplicon-based metagenomics approach (metabarcoding) provides detailed access to the diversity of the mycobiome in any substrate in distinct environments on Earth. Fungi are the main decomposers of lignocellulosic woody debris in terrestrial forested ecosystems, contributing significantly to the global carbon cycle. The main objectives of this study were to assess the fungal taxonomic diversity in fallen woody debris samples from two Neotropical forest fragments (rainforest and seasonal forest), to analyze the qualitative and quantitative components of the taxonomic diversity, and to investigate the functional diversity of the ecological groups detected. Our study comprised three main methodological steps: (i) sampling in the field; (ii) extraction of DNA, amplification of targeted segments and massively parallel sequencing; and (iii) data analysis and interpretation. A total of 110 molecular operational taxonomic units showing sequence similarity of 95% or more across the two collection sites using two DNA metabarcoding markers (ITS1 and ITS2) were assigned to putative fungal genera in 59 families, 27 orders, and 3 phyla. The number of putative fungal genera and the relative abundance of reads for each genus are higher in the tropical rainforest site than in the tropical seasonal forest site. Most of the identified genera are ligninolytic and cellulolytic and/or hemicellulolytic Basidiomycota (Agaricomycetes) and Ascomycota (Sordariomycetes), but “sugar fungi” and fungi associated with plants and detritivorous insects were also detected. This is the first study using NGS as a rapid and large-scale useful strategy to uncover the diversity of wood-decaying fungi in tropical forests.
local.identifier.orcidhttps://orcid.org/0000-0002-3167-6308
local.identifier.orcidhttps://orcid.org/0000-0001-9038-4631
local.identifier.orcidhttps://orcid.org/0000-0002-0602-7867
local.identifier.orcidhttps://orcid.org/0000-0001-7446-1342
local.identifier.orcidhttps://orcid.org/0000-0002-7692-6243
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.295.1.1

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USING Next-Generation Sequencing (NGS) TO UNCOVER DIVERSITY OF WOOD-DECAYING FUNGI IN NEOTROPICAL ATLANTIC FORESTS.pdf
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