Sclareol is a potent enhancer of doxorubicin: evaluation of the free combination and co-loaded nanostructured lipid carriers against breast cancer

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dc.creatorGabriel Silva Marques Borges
dc.creatorJuliana de Oliveira Silva
dc.creatorRenata Salgado Fernandes
dc.creatorÂngelo Malachias de Souza
dc.creatorGeovanni Dantas Cassali
dc.creatorMaria Irene Yoshida
dc.creatorElaine Amaral Leite
dc.creatorAndré Luís Branco de Barros
dc.creatorLucas Antônio Miranda Ferreira
dc.date.accessioned2023-03-10T19:36:47Z
dc.date.accessioned2025-09-09T01:22:39Z
dc.date.available2023-03-10T19:36:47Z
dc.date.issued2019
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1016/j.lfs.2019.116678
dc.identifier.issn0024-3205
dc.identifier.urihttps://hdl.handle.net/1843/50806
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofLife Sciences
dc.rightsAcesso Restrito
dc.subjectCâncer
dc.subjectQuimioterápicos
dc.subjectCompostos químicos
dc.subject.otherDoxorubicin
dc.subject.otherSclareol
dc.subject.otherCo-encapsulation
dc.subject.otherSynergism
dc.subject.otherBreast cancer
dc.subject.otherNanostructured lipid carriers
dc.titleSclareol is a potent enhancer of doxorubicin: evaluation of the free combination and co-loaded nanostructured lipid carriers against breast cancer
dc.typeArtigo de periódico
local.citation.epage13
local.citation.spage1
local.citation.volume232
local.description.resumoAIMS: In this work, it was sought to determine if there was synergism between doxorubicin (DOX), a well-known antineoplastic, and sclareol (SC), a diterpene from natural origin, in breast cancer treatment. Moreover, it was investigated if their co-loading in the same nanocarrier would result in a gain of activity and/or a toxicity diminishment. MAIN METHODS: The synergism of the DOX:SC combination was evaluated in MDA-MB-231 and 4T1 cells. A nanostructured lipid carrier (NLC) co-encapsulating DOX and SC in their synergistic molar ratio was prepared and characterised, in terms of mean diameter, zeta potential, DOX encapsulation efficiency, small angle X-ray scattering, differential scanning calorimetry, and polarised light microscopy for further intravenous administration. The anticancer activity of the combination, free and encapsulated, was evaluated in 4T1-tumour bearing mice. KEY FINDINGS: It was determined that DOX:SC combination at the molar ratio 1:1.9 presents better synergistic anticancer activity than the molar ratio 1:7.5 in vitro. DOX:SC-loaded NLC (NLC-DOX-SC) improved in vitro cytotoxic and in vivo antitumour activity compared to free DOX. Although NLC-DOX-SC and free DOX:SC, at the synergistic molar ratio, showed similar activity in the in vivo study, the free combination provoked body weight loss, behaviour alterations and haematological toxicity in the animals, while this was not observed for NLC-DOX-SC. SIGNIFICANCE: This work shows that SC and DOX present synergistic anticancer activity for breast cancer treatment whereas NLC-DOX-SC was a feasible alternative to attain the benefits posed by DOX:SC combination but with none to fewer side effects.
local.identifier.orcidhttps://orcid.org/0000-0002-7360-8231
local.identifier.orcidhttps://orcid.org/0000-0002-8703-4283
local.identifier.orcidhttps://orcid.org/0000-0002-5650-6743
local.identifier.orcidhttps://orcid.org/0000-0002-6795-9457
local.identifier.orcidhttps://orcid.org/0000-0003-2474-5536
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.departmentICX - DEPARTAMENTO DE FÍSICA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0024320519306046

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