Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis

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dc.creatorAlessandra Rejane Ericsson de Oliveira
dc.creatorAnna Christina de Almeida
dc.creatorCintya Neves Souza
dc.creatorLivia Mara Vitorino da Silva
dc.creatorAdriana Alberico Xavier Ruas
dc.creatorDemerson Arruda Sanglard
dc.creatorAfrânio Farias de Melo Júnior
dc.creatorAlexandre Moisés Ericsson de Oliveira
dc.creatorMauro Aparecido de Sousa Xavier
dc.date.accessioned2022-06-10T15:25:29Z
dc.date.accessioned2025-09-09T01:15:02Z
dc.date.available2022-06-10T15:25:29Z
dc.date.issued2017
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.identifier.doihttps://doi.org/10.4238/gmr16029709
dc.identifier.issn1676-5680
dc.identifier.urihttps://hdl.handle.net/1843/42423
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofGenetics and Molecular Research
dc.rightsAcesso Aberto
dc.subjectGenética
dc.subjectMastite
dc.subjectBovino de leite
dc.subjectStaphylococcus aureus
dc.titlePhenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis
dc.typeArtigo de periódico
local.citation.issue2
local.citation.volume16
local.description.resumoThe Staphylococcus aureus is the most common isolated microorganism in ruminant animal species diagnostic with clinical or subclinical mastitis. Dairy herds with these diseases can transfer S. aureus into the milk supply, which can lead to food poisoning in humans. The objective of this study was to evaluate the profile of antimicrobial susceptibility, the presence of femA gene, the genetic relationships among isolates of S. aureus obtained from milk originating from flocks diagnosed with subclinical mastitis in nine rural properties in the northern of Minas Gerais State. To this end, 498 samples of bovine milk tested positive for the California mastitis test (CMT) were subjected to morphological methods and biochemical patterns for microbiological presumptive identification of S. aureus. The PCR test with the genetic marker femA was used to confirm the species S. aureus. All the 26 isolates presumptively identified as S. aureus amplified a fragment of 132 bp corresponding to the femA gene. The profile of antimicrobial susceptibility was performed according to the disk-diffusion methodology and two isolates were susceptible to all the antibiotics tested. The drug multiresistence was found in 80.76% of the isolates. The determination of the genetic profile and the clonal relationship among the isolates was performed by the method of DNA RAPD-PCR polymorphism. The S. aureus isolates were divided into two groups with 26 distinct subgroups. The analysis of RAPD-PCR showed no genetic diversity among them, heterogeneous profile and absence of clonality.
local.identifier.orcidhttps://orcid.org/ 0000-0001-9836-4117
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://europepmc.org/article/MED/28671260

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