Resposta imune local às lesões hpv induzidas do colo uterino em pacientes portadoras e não portadoras do vírus da imunodeficiência humana

Abstract

The objective of this study was to determine local immunity, by evaluating Langerhans cell density in cervical biopsies of HIV-positive and HIV-negative women. A cross-sectional study that included HIV-infected and HIV-non-infected women. All patients had HPV-DNA in uterine cervix, detected by Polymerase Chain Reaction or Hybrid Capture II, and their cervical biopsies were assessed for Langerhans cell density and intraepithelial neoplasia (CIN). Langerhans cells were idenfied by immunohistochemistry using CD1a and S100 antibodies. Associationsamong cervical Langerhans cell density, cervical histology, TCD4+ cells count and HIV viral load were analyzed by logistic regression (SPSS, version 12.0). 77 women (40 seropositive and 37 seronegative) were enrolled. Langerhans cell density of HIV-infected women was 0.80 ± 0.7 cells (antibody CD1a) and 1.3 ± 1.0 cells (antibody S100), and in the HIV-non-infected women was 2.6 ± 1.6 cells (antibody CD1a) and 3.6 + 1, 7 cells (antibody S100). Univariate analyses showed significant differences between groups: CD1a (p < .0001) and S100 (p < .0001). There was no association among Langerhans cell density and viral load, TCD4+ cells count or human papillomavirus genotypes (p> .05). In a logistic regression model only HIVinfection was independently associated to decrease in Langerhans cell density. HIV-infection is an independent factor to explain the decrease of local immunity of uterine cervix, allowing the development of cervical lesions. This effect is notassociated to TCD4+ cells count or HIV viral load.