Comprehensive probiogenomics analysis of the commensal Escherichia coli CEC15 as a potential probiotic strain

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dc.creatorTales Fernandoda Silva
dc.creatorRafael de Assis Glória
dc.creatorThiago Jesus de Sousa
dc.creatorMonique Ferrary Americo
dc.creatorAndria dos Santos Freitas
dc.creatorMarcus Vinicius Canário Viana
dc.creatorLuís Cláudio Lima de Jesus
dc.creatorLigia Carolina da Silva Prado
dc.creatorNathalie Danie
dc.creatorOlivia Ménard
dc.creatorMarie‑Françoise Coche
dc.creatorDidier Dupont
dc.creatorJulien Jardin
dc.creatorAmanda Dias Borges
dc.creatorSimone Odília Antunes Fernandes
dc.creatorValbert Nascimento Cardoso
dc.creatorBertram Brenig
dc.creatorEnio Ferreira
dc.creatorRodrigo Profeta
dc.creatorFlavia Figueira Aburjaile
dc.creatorRodrigo Dias Oliveira de Carvalho
dc.creatorPhilippe Langella
dc.creatorYves Le Loir
dc.creatorClaire Cherbuy
dc.creatorGwénaël Jan
dc.creatorVasco Azevedo
dc.creatorÉric Guédon
dc.date.accessioned2025-01-22T21:58:05Z
dc.date.accessioned2025-09-09T00:57:57Z
dc.date.available2025-01-22T21:58:05Z
dc.date.issued2023-11-27
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1186/s12866-023-03112-4
dc.identifier.issn1471-2180
dc.identifier.urihttps://hdl.handle.net/1843/79420
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofMicrobiologia BMC
dc.rightsAcesso Aberto
dc.subjectProbióticos
dc.subjectPatologia
dc.subjectSistema gastrointestinal
dc.subjectSistema imunológico
dc.subject.otherProbiotics
dc.subject.otherProbiogenomics
dc.subject.otherEscherichia coli CEC15
dc.subject.otherGenomics
dc.subject.otherEscherichia coli Nissle 1917
dc.subject.otherMucositis
dc.subject.otherImmunomodulation
dc.subject.otherGastrointestinal tract
dc.titleComprehensive probiogenomics analysis of the commensal Escherichia coli CEC15 as a potential probiotic strain
dc.typeArtigo de periódico
local.citation.epage30
local.citation.spage1
local.citation.volume23
local.description.resumoBackground: Probiotics have gained attention for their potential maintaining gut and immune homeostasis. They have been found to confer protection against pathogen colonization, possess immunomodulatory effects, enhance gut barrier functionality, and mitigate inflammation. However, a thorough understanding of the unique mechanisms of effects triggered by individual strains is necessary to optimize their therapeutic efficacy. Probiogenomics, involving high-throughput techniques, can help identify uncharacterized strains and aid in the rational selection of new probiotics. This study evaluates the potential of the Escherichia coli CEC15 strain as a probiotic through in silico, in vitro, and in vivo analyses, comparing it to the well-known probiotic reference E. coli Nissle 1917. Genomic analysis was conducted to identify traits with potential beneficial activity and to assess the safety of each strain (genomic islands, bacteriocin production, antibiotic resistance, production of proteins involved in host homeostasis, and proteins with adhesive properties). In vitro studies assessed survival in gastrointestinal simulated conditions and adhesion to cultured human intestinal cells. Safety was evaluated in BALB/c mice, monitoring the impact of E. coli consumption on clinical signs, intestinal architecture, intestinal permeability, and fecal microbiota. Additionally, the protective effects of both strains were assessed in a murine model of 5-FU-induced mucositis. Results: CEC15 mitigates inflammation, reinforces intestinal barrier, and modulates intestinal microbiota. In silico analysis revealed fewer pathogenicity-related traits in CEC15, when compared to Nissle 1917, with fewer toxin-associated genes and no gene suggesting the production of colibactin (a genotoxic agent). Most predicted antibiotic-resistance genes were neither associated with actual resistance, nor with transposable elements. The genome of CEC15 strain encodes proteins related to stress tolerance and to adhesion, in line with its better survival during digestion and higher adhesion to intestinal cells, when compared to Nissle 1917. Moreover, CEC15 exhibited beneficial effects on mice and their intestinal microbiota, both in healthy animals and against 5FU-induced intestinal mucositis.
local.identifier.orcidhttps://orcid.org/0000-0002-0237-9398
local.identifier.orcidhttps://orcid.org/0000-0002-4607-6364
local.identifier.orcidhttps://orcid.org/0000-0002-6501-9533
local.identifier.orcidhttps://orcid.org/0000-0002-2498-7933
local.identifier.orcidhttps://orcid.org/0000-0002-7017-6437
local.identifier.orcidhttps://orcid.org/0000-0001-7708-3033
local.identifier.orcidhttps://orcid.org/0000-0002-4974-8269
local.identifier.orcidhttps://orcid.org/0000-0001-5304-6561
local.identifier.orcidhttps://orcid.org/0000-0003-0520-9391
local.identifier.orcidhttps://orcid.org/0000-0001-9332-4888
local.identifier.orcidhttps://orcid.org/0000-0001-9428-4494
local.identifier.orcidhttps://orcid.org/0000-0002-6139-5187
local.identifier.orcidhttps://orcid.org/0000-0001-7597-9602
local.identifier.orcidhttps://orcid.org/0000-0002-7635-9656
local.identifier.orcidhttps://orcid.org/0000-0002-1835-0303
local.identifier.orcidhttps://orcid.org/0000-0002-0487-3058
local.identifier.orcidhttps://orcid.org/0000-0002-1067-1882
local.identifier.orcidhttps://orcid.org/0000-0001-5960-4341
local.identifier.orcidhttps://orcid.org/0000-0002-0355-1065
local.identifier.orcidhttps://orcid.org/0000-0002-3088-4199
local.identifier.orcidhttps://orcid.org/0000-0001-8694-3381
local.identifier.orcidhttps://orcid.org/0000-0002-4775-2280
local.identifier.orcidhttps://orcid.org/0000-0002-0901-4447
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA
local.publisher.initialsUFMG
local.url.externahttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03112-4#Abs1

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