Desenvolvimento e validação de uma metodologia para determinação de metais e metaloides em tecidos animais via digestão ácida em micro-ondas e análise por ICP-MS/MS

Abstract

In this work, a method was developed and validated for the determination of thirty-one chemical species in bovine liver samples via microwave acid digestion and determination by ICP-MS/MS. In the method optimization step, the Ultra Turrax® Tube Drive grinder was used as a pre-treatment strategy for the samples and diluted nitric acid for acid digestion in microwave. Validation was conducted following the guidelines of the Ministério da Agricultura, Pecuária e Abastecimento Supply (MAPA) and the evaluated parameters were linearity, selectivity, matrix effect, limit of detection (LD), limit of quantification (LQ), precision, recovery and robustness. The LD and LQ of the method varied from 0.15 to 10 µg L-1 and from 0.045 mg kg-1 to 45 mg kg-1, respectively, the recovery and accuracy of the method varied between 81 and 109% and in the robustness study it was concluded that the method does not undergo significant changes, therefore it is considered robust. Once the validation of the method for a bovine liver sample was completed, experiments were carried out to expand the scope in bovine blood and kidney, porcine liver and kidney, chicken liver, fish liver, muscle and gills. Linearity, selectivity, accuracy and recovery were tested for each new additional matrix and the method is shown to be suitable for determination of metals, metalloids and rare earths in different animal tissues.