The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis

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dc.creatorFlávia Viana Ferreira
dc.creatorEric Roberto Guimarães Rocha Aguiar
dc.creatorRoenick Proveti Olmo
dc.creatorKarla Pollyanna Vieira de Oliveira
dc.creatorEmanuele Guimarães Silva
dc.creatorMaurício Roberto Viana Sant’Anna
dc.creatorNelder de Figueiredo Gontijo
dc.creatorErna Geessien Kroon
dc.creatorJean Luc Imler
dc.creatorJoão Trindade Marques
dc.date.accessioned2025-02-07T21:15:41Z
dc.date.accessioned2025-09-09T00:08:43Z
dc.date.available2025-02-07T21:15:41Z
dc.date.issued2018-06-04
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0006569
dc.identifier.issn1935-2735
dc.identifier.urihttps://hdl.handle.net/1843/79770
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPLOS Neglected Tropical Diseases
dc.rightsAcesso Aberto
dc.subjectInfecções por Arbovirus
dc.subjectInterferência de RNA
dc.subjectRNA Interferente Pequeno
dc.subjectArboviroses
dc.titleThe small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis
dc.typeArtigo de periódico
local.citation.epage19
local.citation.issue6
local.citation.spage01
local.citation.volume12
local.description.resumoSandflies are well known vectors for Leishmania but also transmit a number of arthropod-borne viruses (arboviruses). Few studies have addressed the interaction between sandflies and arboviruses. RNA interference (RNAi) mechanisms utilize small non-coding RNAs to regulate different aspects of host-pathogen interactions. The small interfering RNA (siRNA) pathway is a broad antiviral mechanism in insects. In addition, at least in mosquitoes, another RNAi mechanism mediated by PIWI interacting RNAs (piRNAs) is activated by viral infection. Finally, endogenous microRNAs (miRNA) may also regulate host immune responses. Here, we analyzed the small non-coding RNA response to Vesicular stomatitis virus (VSV) infection in the sandfly Lutzoymia longipalpis. We detected abundant production of virus-derived siRNAs after VSV infection in adult sandflies. However, there was no production of virus-derived piRNAs and only mild changes in the expression of vector miRNAs in response to infection. We also observed abundant production of virus-derived siRNAs against two other viruses in Lutzomyia Lulo cells. Together, our results suggest that the siRNA but not the piRNA pathway mediates an antiviral response in sandflies. In agreement with this hypothesis, pre-treatment of cells with dsRNA against VSV was able to inhibit viral replication while knock-down of the central siRNA component, Argonaute-2, led to increased virus levels. Our work begins to elucidate the role of RNAi mechanisms in the interaction between L. longipalpis and viruses and should also open the way for studies with other sandfly-borne pathogens.
local.identifier.orcidhttps://orcid.org/0000-0001-8716-6828
local.identifier.orcidhttps://orcid.org/0000-0002-8143-5756
local.identifier.orcidhttps://orcid.org/0000-0002-3849-8591
local.identifier.orcidhttps://orcid.org/0000-0002-2504-0979
local.identifier.orcidhttps://orcid.org/0000-0003-3357-2497
local.identifier.orcidhttps://orcid.org/0000-0003-2721-3826
local.identifier.orcidhttps://orcid.org/0000-0003-0740-8319
local.identifier.orcidhttps://orcid.org/0000-0002-3457-3320
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0006569&type=printable

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